Ebook Practical manual of medical microbiology: Part 2
➤ Gửi thông báo lỗi ⚠️ Báo cáo tài liệu vi phạmNội dung chi tiết: Ebook Practical manual of medical microbiology: Part 2
Ebook Practical manual of medical microbiology: Part 2
Nitrate Beducticn TestPRINCIPLEThis test detects the presence of enzyme nitrate reductase whichcauses the reduction of nitrate to nitrite in the prese Ebook Practical manual of medical microbiology: Part 2ence of a suitableelectron donor. Almost all Enterooscreriaceae madzers reduce nitrate.Nitrate reduction can be detected by an appropriate colorimetricRequirement1MediumPotassium nitrate - 0.2 gmPeptone-5 gmDistilled water - 1 litreTube in 5 ml amounts and autoclave at 121°c for 15 min.2Test reagent Ebook Practical manual of medical microbiology: Part 2Solution A- Dissolve 8 gn sulphanilic acid in 1 litre ofacetic acid 5 mol/litSolution B- Dissolve 5.0 gn of Obnaphthylamir^ in litreNitrate Redu^.,MetEbook Practical manual of medical microbiology: Part 2
hod•Inoculate the medium with test organism•Incubate at 37 °C for 96 hours.•Ad 0.1 ml of the test reagent to the test culture.ResultA red color vã thiNitrate Beducticn TestPRINCIPLEThis test detects the presence of enzyme nitrate reductase whichcauses the reduction of nitrate to nitrite in the prese Ebook Practical manual of medical microbiology: Part 2tPRINCIPLESome gram negative bacillii produce H2S by breaking down sulphur containing amino acids like cysteine or cystine. Hydrogen sulphide is demonstrated by its ability to form black insoluble sulphides.Method 1There are different methods and media are used for detection H?s production.List of m Ebook Practical manual of medical microbiology: Part 2edia used for H2S detection1Bismuth sulfite agar.2Triple sugar iron agar (TSI).3Lysine iron agar.4Lead acetate agar.Nitrate Beducticn TestPRINCIPLEThis test detects the presence of enzyme nitrate reductase whichcauses the reduction of nitrate to nitrite in the preseNitrate Beducticn TestPRINCIPLEThis test detects the presence of enzyme nitrate reductase whichcauses the reduction of nitrate to nitrite in the preseGọi ngay
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