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HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

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Nội dung chi tiết: HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSIONIHC responsible for the methods:Stefan Bogdanov Swiss Bee Research CentreFAM, Liebefeld. CH-30

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS 003 Bern, Switzerlandsend all feed-back to: Stefan.bogdanov@fam.admin.chINTRODUCTION AND GENERAL COMMENTS ON THE METHODSMany of the present official h

oney analysis methods and thus also the regulatory norms based on them, are outdated and need revision (1). A Commission was formed in 1990 to carry o HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

ut this task. Stefan Bogdanov. Switzerland, is ex-chairman the IHC and responsible for compiling the methods. Werner der Ohe. Germany is present chair

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

man, and Peter Martin. UK is secretary.The present selection of methods has been made to include all those which at the present state of knowledge are

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSIONIHC responsible for the methods:Stefan Bogdanov Swiss Bee Research CentreFAM, Liebefeld. CH-30

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS e modem ones. All the methods compiled in this publication have been published previously and with one exception (specific rotation) have been subject

ed to collaborative study. Most of them have also been through the standardisation procedure of the German Institute for Norms (DIN).The methods were HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

originally published in 1997 in "Apidologie" (2). As the methods are currently improved, changes to these methods have been made since then, which, ho

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

wever, should improve the methods. Thus, it is the aim of this Internet publication to make the latest version of the IHC methods available to as many

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSIONIHC responsible for the methods:Stefan Bogdanov Swiss Bee Research CentreFAM, Liebefeld. CH-30

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS n freely by reached also by other Homepages by making a link to the Homepage of the IHC. No changes can be made without the approval of the IHC.Additi

ons and remarks to the methods, made after the Apidologie publication:Determination of humidity by digital refractometry (Method 1) and determination HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

of invertase (Method 10): decided at the Dijon meeting in October 1999.Determination of HMF by HPLC (Method 5.1): change of the sample preparation.Col

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

laborative trials and Precision of the methodsThe precision data for the methods have been compiled from 3 sources:a)the original DIN methods, which u

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSIONIHC responsible for the methods:Stefan Bogdanov Swiss Bee Research CentreFAM, Liebefeld. CH-30

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS carried out by the International Honey Commission. These have been interpreted by the more modern robust method of statistics(6-8).1The ISO method ha

s the drawback that outlier laboratories are eliminated from the computation of the precision parameters and also has other drawbacks (6). The robust HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

method, introduced recently, does not have the drawbacks of the ISO methods and takes into account the results of all laboratories taking part in the

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

trial. It is gaining increasing acceptance. In the new edition of the guide for conducting collaborative studies (reference 4. currently in revision)

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSIONIHC responsible for the methods:Stefan Bogdanov Swiss Bee Research CentreFAM, Liebefeld. CH-30

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS determined parameter and the two precision parameters, repeatability r and reproducibility R, as these are the crucial parameters for evaluation of th

e precision of a method.Repeatability rThe difference between the results of two determinations, obtained in rapid succession by the same method on id HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

entical test material under the same conditions ( same operator, same apparatus, same laboratory) shall not exceed the values given in the precision t

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

ablesReproducibility RThe difference between the results of two independent determinations, obtained by the same method on identical test material und

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSIONIHC responsible for the methods:Stefan Bogdanov Swiss Bee Research CentreFAM, Liebefeld. CH-30

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS h measurements are valid with a probability of 95 %. This means that on average in carrying out 20 determinations. 1 outlier may be expected.The inter

laboratory variation, determined as the coefficient of variation of R or RSDr % is the quality parameter most often used to compare the precision of a HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

nalytical methods (8). RSDr % is calculated as: 100 R/x 2.8Generally RSD % decreases exponentially with increasing concentration of the measured varia

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

ble. In the methods for analysis of major foodstuff components, which lie between 0.01 g/100 g and 10 g/100 g, the coefficient of variation will mostl

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSIONIHC responsible for the methods:Stefan Bogdanov Swiss Bee Research CentreFAM, Liebefeld. CH-30

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS methods we have summarised the extreme RSDr % values for each method from the collaborative studies (table 1).International Honey Commission (2002)2M

ethod, parameterRSDr % MIN-MAX1. Moisture, refractometry1.0-2.52. Electrical conductivity3.4-4.43. Ash content4.3-13.24. Free acidity by titration to HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSION INTRODUCTION AND GENERAL COMMENTS ON THE METHODS

pH 8.3 (2)10.3-22.05. Free acidity (2) with equivalence point titration8.7-46.86. Hydroxymethylfurfural with HPLC6.1-10.9

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSIONIHC responsible for the methods:Stefan Bogdanov Swiss Bee Research CentreFAM, Liebefeld. CH-30

HARMONISED METHODS OF THE INTERNATIONAL HONEY COMMISSIONIHC responsible for the methods:Stefan Bogdanov Swiss Bee Research CentreFAM, Liebefeld. CH-30

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