Microwire arrays for studying the influence of temperature gradients on the cellular behavior
➤ Gửi thông báo lỗi ⚠️ Báo cáo tài liệu vi phạmNội dung chi tiết: Microwire arrays for studying the influence of temperature gradients on the cellular behavior
Microwire arrays for studying the influence of temperature gradients on the cellular behavior
TimFakultat fur Elektrotechnik und InformationstechnikProfessor fur NeuroelektronikMicrowire arrays for studying the influence of temperature gradient Microwire arrays for studying the influence of temperature gradients on the cellular behavior ts on the cellular behaviorTruong Ka My DangVollstăndiger Abdruck der von der Eakultăt fur Elektrotechnik und Informationstechnik der Technischen Universităt Munchen zur Erlangung des akademischen Grades einesDoktor-lngenieurs (Dr.-lng.)genehmigten Dissertation.Vorsitzende: Prof. Dr.-lng.Christian J Microwire arrays for studying the influence of temperature gradients on the cellular behavior irauschekPrufer der Dissertation:1Prof. Dr. Bernhard Wolfrum2Prof. Dr. Oliver HaydenDie Dissertation wurde am 13.08.2018 bei der Technischen UniversitMicrowire arrays for studying the influence of temperature gradients on the cellular behavior
ãt Mũnchen eingereicht und durch die Fakultat fur Elektrotechnik und Informationstechnik am 03.12.2018 angenommen.AbstractTemperature plays an importaTimFakultat fur Elektrotechnik und InformationstechnikProfessor fur NeuroelektronikMicrowire arrays for studying the influence of temperature gradient Microwire arrays for studying the influence of temperature gradients on the cellular behavior nabled highly localized temperature control at the scale of a few microns. This allows the study of thermal influences on biological microsystems. In this context, the present thesis investigates the changes of cellular behavior depending on temperature gradients using microwire arrays. In addition, Microwire arrays for studying the influence of temperature gradients on the cellular behavior the thesis also introduces a sacrificial layer approach to fabricate microchannels for studying neuronal guidance. The integration of the microwire aMicrowire arrays for studying the influence of temperature gradients on the cellular behavior
rray into microchannels presented in this thesis proposes a novel method for governing neuronal networks under the influences of temperature gradientsTimFakultat fur Elektrotechnik und InformationstechnikProfessor fur NeuroelektronikMicrowire arrays for studying the influence of temperature gradient Microwire arrays for studying the influence of temperature gradients on the cellular behavior tion in cardiac cell networks. In order to investigate the effects of temperature on cellular networks, a monolayer of cardiomyocytes is cultured onto the chip. The localized temperature is induced by using a power supply connected to microwire arrays. A calcium imaging method is performed to evalua Microwire arrays for studying the influence of temperature gradients on the cellular behavior te changes in signal propagation under local heat Stimulation. Calcium signals are recorded using a high speed and low light camera. Also in this partMicrowire arrays for studying the influence of temperature gradients on the cellular behavior
, the velocity of the calcium signal propagation in cellular networks is locally increased upon heat stimulation. Additionally, this part presents a rTimFakultat fur Elektrotechnik und InformationstechnikProfessor fur NeuroelektronikMicrowire arrays for studying the influence of temperature gradient Microwire arrays for studying the influence of temperature gradients on the cellular behavior th of neurons is investigated under the influence of temperature gradients. In this study, the PC 12 neuron-like cells are cultured on the microwire array chip. The effects of temperature gradients on neurons are examined, focusing on the speed and direction of neurite outgrowth. The growth of neuri Microwire arrays for studying the influence of temperature gradients on the cellular behavior tes under local heat stimulation is recorded using a time-lapse imaging method. The images are then analyzed to estimate the development of neurons wiMicrowire arrays for studying the influence of temperature gradients on the cellular behavior
thin temperature gradients. The result indicates that the growth speed of neurons increases with heat stimulation. Moreover, the evidence that temperaTimFakultat fur Elektrotechnik und InformationstechnikProfessor fur NeuroelektronikMicrowire arrays for studying the influence of temperature gradient Microwire arrays for studying the influence of temperature gradients on the cellular behavior resents a promising fabrication approach, based on standard clean room fabrication and sacrificial layer etching, for combining microwire arrays with a set of perpendicular axon-guiding microchannels. This approach enables the positioning of neurites, as well as control over their polarity. In parti Microwire arrays for studying the influence of temperature gradients on the cellular behavior cular, an array of asymmetrical microchannels with wide inlets and narrow outlets is fabricated to allow neurites to grow in one direction. The experiMicrowire arrays for studying the influence of temperature gradients on the cellular behavior
ment with neu rites from PC12 cells demonstrates that neurites can grow from the entrance to the exit of the microchannels. Furthermore, the fabricatiTimFakultat fur Elektrotechnik und InformationstechnikProfessor fur NeuroelektronikMicrowire arrays for studying the influence of temperature gradient Microwire arrays for studying the influence of temperature gradients on the cellular behavior rks.Overall, this thesis focuses on investigating thermal impacts on cellular behavior using the localized heating generated by microwire arrays. This work demonstrates the important role of microscopic heating in regulating signal propagation, as well as the growing properties of cellular networks. Microwire arrays for studying the influence of temperature gradients on the cellular behavior Specifically, the temperature highly confined in a small area governs the calcium signal propagation of cardiomyocytes and the growth of neurites towMicrowire arrays for studying the influence of temperature gradients on the cellular behavior
ards the heat source. Other than that, concerning the technical aspects, the fabrication of thin microwire enables US to control high temperature in aTimFakultat fur Elektrotechnik und InformationstechnikProfessor fur NeuroelektronikMicrowire arrays for studying the influence of temperature gradient Microwire arrays for studying the influence of temperature gradients on the cellular behavior fabrication steps and improving the adhesion between microchannels and the chip's surface.iiiivTimFakultat fur Elektrotechnik und InformationstechnikProfessor fur NeuroelektronikMicrowire arrays for studying the influence of temperature gradientGọi ngay
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