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Taxonomic characterization of an acidophilic bacterium

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Nội dung chi tiết: Taxonomic characterization of an acidophilic bacterium

Taxonomic characterization of an acidophilic bacterium

Taxonomic characterization of an acidophilic bacterium isolated from the acidophilic nitrifying process2009MASTER OF ENGINEERINGNGUYEN HƯYEN THI THANH

Taxonomic characterization of an acidophilic bacterium HStudent Number: M085807TOYOHASHI UNIVERSITY OF TECHNOLOGY1Taxonomic characterization of an acidophilic bacterium isolated from the acidophilic nitrif

ying processAbstract.Nitrification and denitrification processes in which ammonia in municipal and industrial wastewater is converted into nitrogen by Taxonomic characterization of an acidophilic bacterium

nitrifying and denitrifying microorganisms, are the centre of nitrogen cycle. Nitrification and denitrification at acidophilic condition is considere

Taxonomic characterization of an acidophilic bacterium

d as a prospective solution for treatment wastewater containing amonia. Toward this purpose, our laboratory has designed an operator ANSBR (Acidophili

Taxonomic characterization of an acidophilic bacterium isolated from the acidophilic nitrifying process2009MASTER OF ENGINEERINGNGUYEN HƯYEN THI THANH

Taxonomic characterization of an acidophilic bacterium in this operator, we have studied bacterial community structure and isolated one strain that was first identified as the genus Nitrobacrer (reidentif

ied to the genera Dỵeììá). Tn this study; a new strain isolated from ANSBR (Acidophilic Nitrifying sequencing Balch Reactor) were characterized taxono Taxonomic characterization of an acidophilic bacterium

mically. The strain were characterized using phenotypic analysis and molecular based methods. Physiological lest and quinone analysis were carried out

Taxonomic characterization of an acidophilic bacterium

as well as 16S rRNA sequence. Using 16S rRNA gene sequence analysis and phylogenetic tree showed this strain was similar to the genus Dyetta, Fralett

Taxonomic characterization of an acidophilic bacterium isolated from the acidophilic nitrifying process2009MASTER OF ENGINEERINGNGUYEN HƯYEN THI THANH

Taxonomic characterization of an acidophilic bacterium systems (Kevin el al.. 2005). As the result, our strain is Gram-negative, motile, could grow at pH 4.0 8.0 and optimum pH was 5.0. This strain grew s

lowly al 10 or 55°c. and optimum temperature for for growth was 25yc. This strain could grow at R2A medium, and very well at NA medium but could not g Taxonomic characterization of an acidophilic bacterium

row at the medium that used for Frateuria, Acetobacier and Ghtcenobacter. Therefore, based on medium lest dalas, we found this strain was not the genu

Taxonomic characterization of an acidophilic bacterium

s Frateuria that can produce a water-soluble brown pigment on glucose-y-east extract-CaCCb, and use AF. broth the medium was used to identified the ge

Taxonomic characterization of an acidophilic bacterium isolated from the acidophilic nitrifying process2009MASTER OF ENGINEERINGNGUYEN HƯYEN THI THANH

Taxonomic characterization of an acidophilic bacterium e aim is to provide the first general characteristics of the strain isolated from activated sludge for the further intensive research, particularly G/

C content and fatty acid analysis, two powerful tools to identified strains at species level.2ContentChapter 1. introduction about acidophilic nitrify Taxonomic characterization of an acidophilic bacterium

ing process and microorganism population in this process1.1.General introduction about nitrifying process................................51.2.The inve

Taxonomic characterization of an acidophilic bacterium

ntion of acidophilic nitrifying process..............................61.3.The genus Dyeìỉa, Fraieuria and Rhodanobacter...............................

Taxonomic characterization of an acidophilic bacterium isolated from the acidophilic nitrifying process2009MASTER OF ENGINEERINGNGUYEN HƯYEN THI THANH

Taxonomic characterization of an acidophilic bacterium roduction ..............................................................102.2.Materials and methods...................................................

...122.2.1.Test strain.......................................................122.2.2.Test growth medium .............................................. Taxonomic characterization of an acidophilic bacterium

.122.2.3.Cell morphologyand cultural characteristic.......................122.2.4.pH test...........................................................12

Taxonomic characterization of an acidophilic bacterium

2.2.5.Temperature test..................................................132.2.6.NaCI tolerance test...............................................132.

Taxonomic characterization of an acidophilic bacterium isolated from the acidophilic nitrifying process2009MASTER OF ENGINEERINGNGUYEN HƯYEN THI THANH

Taxonomic characterization of an acidophilic bacterium Results ...................................................................162.3.1.Test strain and cultivation.......................................1

62.3.2.Test growth medium ...............................................172.3.3.Cell morphology and cultural characteristic.......................182 Taxonomic characterization of an acidophilic bacterium

.3.4.pll test..........................................................192.3.5.Temperature test..................................................202.3

Taxonomic characterization of an acidophilic bacterium

.6.NaCl tolerance test...............................................212.3.7.Growth measurement................................................22

Taxonomic characterization of an acidophilic bacterium isolated from the acidophilic nitrifying process2009MASTER OF ENGINEERINGNGUYEN HƯYEN THI THANH

Taxonomic characterization of an acidophilic bacterium isolated from the acidophilic nitrifying process2009MASTER OF ENGINEERINGNGUYEN HƯYEN THI THANH

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