Assessment of the methods for the detection of hop stunt virus and yellow speckle virus related grapevine

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Assessment of the methods for the detection of hop stunt virus and yellow speckle virus related grapevine

https://khothu vien .comDAYEH UNIVERSITYENVIRONMENTAL ENGINEERING DEPARTMENTCOLLEGE OF ENGINEERINGAssessment of the Methods for the Detection of Hop S

Assessment of the methods for the detection of hop stunt virus and yellow speckle virus related grapevine Stunt Virus and Yellow Speckle Virus Related GrapevineStudent: Nguyen Phuc ThienAdvisor: Chen Yi ChingTAIWAN 106-6-30* t X * í« 1* X ® > Ậ tặ i ỈUK.ị8

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ỉ- MMu t H fò % ZOlIL JiS-}ỴMl^()t < a 106^6^ 30 aABSTRACTIn this study a multiplexreverse-transcription polymerase chain reaction (mRT-PCR) technique

Assessment of the methods for the detection of hop stunt virus and yellow speckle virus related grapevine

is used simultaneously to detect viroids in grapevine. Fifty grapevine leaf samples with yellow ing or mosaic symptoms were collected from difference

https://khothu vien .comDAYEH UNIVERSITYENVIRONMENTAL ENGINEERING DEPARTMENTCOLLEGE OF ENGINEERINGAssessment of the Methods for the Detection of Hop S

Assessment of the methods for the detection of hop stunt virus and yellow speckle virus related grapevine apevine viroid (AGVd). Grapevine yellow speckle vjroid-l (GYSVd-1), Grapevine yellow speckle viroid-2 (GYSVd-2) and Citrus exocorlis viroid (CliVd) we

re selected from previous reports or primers were newly designed according to the sequences obtained from NCBĨ Genbank. Comparison of the sequence ide Assessment of the methods for the detection of hop stunt virus and yellow speckle virus related grapevine

ntity of HSVd-DY with other HSVd from GcnBank ranged from 37.6% Io 99.7%. While the sequence identity between GYSVd-1 -DY with others ranged from 82.9

Assessment of the methods for the detection of hop stunt virus and yellow speckle virus related grapevine

% to 99.7%. A phylogenetic tree derived from the HSVd sequence indicated that HSVd-DY was closer to an Tran isolate (Ki 916041). while GYSVd-l-DY was

https://khothu vien .comDAYEH UNIVERSITYENVIRONMENTAL ENGINEERING DEPARTMENTCOLLEGE OF ENGINEERINGAssessment of the Methods for the Detection of Hop S

Assessment of the methods for the detection of hop stunt virus and yellow speckle virus related grapevine £401« $ Ỷ ốù^# ’ £ 20152016 >#) 5 ỉ. ố $aUHl^iÊ&A ’ OMCBI, >mutỏow(HSVd) %4 (AGVd) .(GYSVd-1) x O#Mfcỉi-&-2(GYSVd-2)J -Ế-tLlzĩ4ỷ'Ỉ^^^Ã ’(IISVd-DY) &;

OJ£ỏW^4b4ML&^(HSVd)37.6%-99.7% o iTo^íỉ, SJ#-1 (GYSVd-l-DY)82.9%-99.7% o ịt^irS^^ÍL(HSVd)(GYSVd-l-ĐY)(KE916041) ’^.^-^-1 (GYSVd-l-ĐY)(.IE746I88)sa <•j Assessment of the methods for the detection of hop stunt virus and yellow speckle virus related grapevine

£Oh ẩoiệ ■S-íí£^^4-1.

https://khothu vien .comDAYEH UNIVERSITYENVIRONMENTAL ENGINEERING DEPARTMENTCOLLEGE OF ENGINEERINGAssessment of the Methods for the Detection of Hop S