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Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

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Nội dung chi tiết: Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

THESISCHARACTERIZATION or PROBIOTICS Bacillus spp. ISOLATED FROM STRIPED CATFISH [Pangasianodon hypophthalmus (Sauvage, 1878)] AND THE APPLICATION AS

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement FEED SUPPLEMENTHO TH I TRUONG THYGRADUATE SCHOOL, KASETSART UNIVERSITY2016THESISCHARACTERIZATION OF PROBIOTICS Bacillus spp. ISOLATED FROM STRIPED CA

TFISH IPưngưsianodon hypophthalntus (Sausage. 1878)1 AND THE APPLICATION AS FEED SUPPLEMENTHO TH I TRUONG THYA Thesis Submitted in Partial Fulfillment Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

of the Requirements for the Degree of Doctor of Philosophy (Aquaculture) Graduate School. Kasetsart University 2016Ho Tlii Truong Thy 2016: Character

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

ization of Probiotics Bacillus spp. Isolated from Striped (?atfĩsh I I’angasianodon hypophthalmus (Sauvage, 1 S7X)| and the Application as Feed Supple

THESISCHARACTERIZATION or PROBIOTICS Bacillus spp. ISOLATED FROM STRIPED CATFISH [Pangasianodon hypophthalmus (Sauvage, 1878)] AND THE APPLICATION AS

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement Ph D. 132 pages.Identification of 6 species of Bacillus (B. sublilis, B. amyloliquejaciens. B. cercus. B. megateriiun. B. licheniformis and B. puniil

us) by PCR technique was developed to identify 120 isolates collected from striped catfish farms in 3 provinces in the southern part of Vietnam: An Gi Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

ang. Dong Thap. and Can Tho. The highest number of Bacillus population collected from the three provinces was B. megaterium and there was no isolate i

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

dentified as B. licheniformes. The two Bacillus spp. chosen for in vivo experiments were 54A-B. amyloliquefaciens (BA) with high protease activity and

THESISCHARACTERIZATION or PROBIOTICS Bacillus spp. ISOLATED FROM STRIPED CATFISH [Pangasianodon hypophthalmus (Sauvage, 1878)] AND THE APPLICATION AS

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement le salt condition after 3 h and 24 II of exposure. Evaluation of antibiotic susceptibility showed (hat all six probiotics candidates were susceptible

to all antibiotics except spectinomycin. oxytetracycline, and sulfamethoxazole.Three doses of mixed probiotics spore of BA and BP were applied in stri Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

ped catfish feed at I X IO8. 3x IO8, and 5xl()8 CFU/g feed. Although there were no significant effects on % SGR and FCR (F>0.05) of fish. AWG was sign

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

ificantly higher in fish fed with 5x 108 CFU/g feed compared to the control (P<0.05). Similarly, the significant increase of lysozyme, phagocytic acti

THESISCHARACTERIZATION or PROBIOTICS Bacillus spp. ISOLATED FROM STRIPED CATFISH [Pangasianodon hypophthalmus (Sauvage, 1878)] AND THE APPLICATION AS

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement more resistance offish against Ewardsiella iclaluri infection during 15 days of challenge. All doses of probiotics stimulated significantly higher st

ress tolerance to ammonia concentration at 150 ppm I AN.Student's signatureThesis advisor’s signaturehttps://khothuvien.cori!ACKNOWLEDGEMENTSFirst of Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

all. I want to express sincere gratitude to my thesis advisor: Associate Professor Nontauith Areechon. Ph.D. and my committee members: Assistant Profe

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

ssor Sasimanas Unajak, Ph D. and Assistant Professor Ruangvit Yoonpundh, Ph D., for their suggestion and encouragement during the period of my study i

THESISCHARACTERIZATION or PROBIOTICS Bacillus spp. ISOLATED FROM STRIPED CATFISH [Pangasianodon hypophthalmus (Sauvage, 1878)] AND THE APPLICATION AS

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement nal referee for my thesis defense examination.I also give thanks to my sponsor. Ministry of Agriculture and Rural Development, for the financial suppo

rt of my PI).D. study.Next, I should not forget to thank the fish fanners at Me Kong Delta who provided the necessary information and the samples for Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

this study, as well as sincerely gratitude to Dr. Nguyen Him Thinh and Assistant Professor Nguyen Nhu Tri, Ph D. from Faculty of Fisheries. Nong Lam U

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement

niversity for their suggestions and assistances.Finally, I am grateful to express my acknowledgment to my parent and my husband who give me support, l

THESISCHARACTERIZATION or PROBIOTICS Bacillus spp. ISOLATED FROM STRIPED CATFISH [Pangasianodon hypophthalmus (Sauvage, 1878)] AND THE APPLICATION AS

Characterization of probiotics bacillus spp isolated from striped catfist pangasianodon hypophthalmus (sauvage, 1878) and the application as feed supplement IST OF FIGURESivINTRODUCTION1OBJECTIVES4LITERATURE REVIEWS5MATERIALS AND METHODS32Materials32Methods36RESULTS AND DISCUSSION54

THESISCHARACTERIZATION or PROBIOTICS Bacillus spp. ISOLATED FROM STRIPED CATFISH [Pangasianodon hypophthalmus (Sauvage, 1878)] AND THE APPLICATION AS

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