Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization
➤ Gửi thông báo lỗi ⚠️ Báo cáo tài liệu vi phạmNội dung chi tiết: Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization
Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization
UNIVERSITY OF LIEGE GENETICS AND PHYSIOLOGY OF MICROALGAE LABORATORY* liegeuniversitéCharacterization of Vietnamese microalgae strains for aquaculture Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization e wastewater treatment and biomass valorizationn, ® o®o ; o"HU - liililKiRwl (?"' va I r__ .<■*)I© : °■ V. 20LUU THAO NGUYENA thesis submitted in fulfillment of the requirements for the degree of Doctor in SciencesAcademic year: 2019 - 2020Promoter: Prof. Claire RemacleCo - promoter: Prof. Gauthier Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization Eppe1AcknowledgementMy gratitude firstly goes to Académie de Recherche et d'Enseignement Supérieur (ARES-CCD, Brussels, Belgium 1 for its financial suCharacterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization
pport during my thesis under the project "RENEWABLE". The research was performed in the laboratories of Genetics and Physiology of Microalgae (InBios.UNIVERSITY OF LIEGE GENETICS AND PHYSIOLOGY OF MICROALGAE LABORATORY* liegeuniversitéCharacterization of Vietnamese microalgae strains for aquaculture Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization City.1 would like lo express my deepest gratitude to Prof. Claire Remade and Prof. Gauthier Eppc for then dedicated supervision. This work could not be completed without their valuable guidance as well as scientific orientation. 1 also want to thank Prof. Le Hung Anil for his great support during m Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization y study ill the laboratory of Institute of Environmental Science. Engineering and Management.Industrial University of Ho Chi Minh City. Prof Spiros AgCharacterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization
athos. partner of the RENEWABLE project, is also acknowledged for his careful reading of the draft of the manuscript.I would like to thank all of my cUNIVERSITY OF LIEGE GENETICS AND PHYSIOLOGY OF MICROALGAE LABORATORY* liegeuniversitéCharacterization of Vietnamese microalgae strains for aquaculture Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization riences.Last but not least. I am indebted to my family members, especially my mom and my wife, who help me take care of my two loved children during my study in Liege. Belgium.2SummaryThe study aims to isolate microalgal strains from shrimp - farm wastewater and select some of them suitable for prod Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization uction of biomass, biodiesel and other valuable products. We have isolated 4 isolates, namely NL3, NL4, NL6 and NLỈ2. The four isolates were preliminaCharacterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization
rily identified using molecular techniques based on partial sequence of 1SS rRNA gene and classified into four different genera belonging to two phylaUNIVERSITY OF LIEGE GENETICS AND PHYSIOLOGY OF MICROALGAE LABORATORY* liegeuniversitéCharacterization of Vietnamese microalgae strains for aquaculture Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization h, protein content, pigment and fatty acid content and profiles. As a result, isolates NL3 and NL6 were chosen for the project. The two strains were further identified based on the whole sequence of 18S rDNA - ITS1-5.8S-ITS2. Isolate NL3 was identified as Desmodesmits sp. NL3. which is proposed to b Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization e a new species with two group I introns (S516 and S1046) while isolate n!6 was 100% identical to Nannoch!or opsis salina (DI 2. accession number JX 1Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization
85299.1) from Shandong in China, so named Nannochloropsis salina NL6. Those two strains were further characterized in terms of growth, protein, pigmenUNIVERSITY OF LIEGE GENETICS AND PHYSIOLOGY OF MICROALGAE LABORATORY* liegeuniversitéCharacterization of Vietnamese microalgae strains for aquaculture Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization ropsis salina NL6 was characterized by a high percentage of fatty acids (40% DW at the end of exponential phase) and a fatty acid profile suitable for biodiesel production. Desmodesmus sp. A’Z.3 was characterized by high protein (40% DW). biomass (1.54 g'L). and lutein (7 mg'g DW) contents at the en Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization d of the exponential phase. Notably, NannocMoropsis salina NL6 (N. salina NL6) was able to produce eicosapentaenoic acid (EPA) and astaxanthin. TheirCharacterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization
performance on the production of these compounds did not change much across the four salinities. The two strains, therefore, fit the project goals.KeyUNIVERSITY OF LIEGE GENETICS AND PHYSIOLOGY OF MICROALGAE LABORATORY* liegeuniversitéCharacterization of Vietnamese microalgae strains for aquaculture Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization icroalgae and classification141.2.1.General information on microalgac141.2.2.Presentation of algae with commercial interest161.2.2.1.Chlorophyla (Green algae».................................................161.2.2.2.Rhodophyta (Red algae)....................................................161.2.2.3 Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization .Bacillariophyta (diatoms).................................................171.2.2.4.Eustigmatophyta..................................................Characterization of vietnamese microalgae strains for aquaculture wastewater treatment and biomass valorization
.........1813Algal cultivation system201.3.1.Closed photobioreactors (PBRs)20UNIVERSITY OF LIEGE GENETICS AND PHYSIOLOGY OF MICROALGAE LABORATORY* liegeuniversitéCharacterization of Vietnamese microalgae strains for aquacultureUNIVERSITY OF LIEGE GENETICS AND PHYSIOLOGY OF MICROALGAE LABORATORY* liegeuniversitéCharacterization of Vietnamese microalgae strains for aquacultureGọi ngay
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