Effects of genotype and environment on wheat starch properties
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Effects of genotype and environment on wheat starch properties
EFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of Docto Effects of genotype and environment on wheat starch properties or of PhilosophyFaculty of Agriculture and Environment The University of Sydney2013Statement of originality1, Minh Tri NHAN, declare that this submission is my own work and that, to the best of my knowledge and belief, this work doesn’t contain any material previously published or written by another Effects of genotype and environment on wheat starch properties person without due acknowledgment, nor has it been submitted for an award of any other degree or qualification of any University or other institute oEffects of genotype and environment on wheat starch properties
f higher learning.Minh Tri NIIANDate: 19 March 2013iiAcknowledgementsI would like to acknowledge and extend my heartfelt gratitude to Professor Les CoEFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of Docto Effects of genotype and environment on wheat starch properties edge scholarships from Vietnam International Education Development. The University of Sydney World Scholars Program, Christian Rowe Thornett Bequest, and Postgraduate Research Support Scheme of The University of Sydney.I am thankful to the Grain Research and Development Corporation Australian, and t Effects of genotype and environment on wheat starch properties he National Variety Trials for providing wheat grain samples and agronomic data.I am grateful for assistance from the staff and use of facilities of tEffects of genotype and environment on wheat starch properties
he Australian Centre for Microscopy and Microanalysis (Scanning electron microscope), and School of Chemistry (Differential Scanning Calorimetry). I eEFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of Docto Effects of genotype and environment on wheat starch properties and Dr. James Doutch for their assistance and to the Bragg Institute. Australian Nuclear Science and Technology Organisation for access to Small Angle X-ray Scattering instrument.I thank the staff in the Faculty of Agriculture and Environment at The University of Sydney for good support. I appreciat Effects of genotype and environment on wheat starch properties e kind advice and help from Dr. Meredith Wilkes. Dr. Thomas Roberts. Dr. Bob Caldwell. Dr. Edith Lees. Dr. Shujun Wang. Ms. Iona Gyorgy, Ms Pamela SteEffects of genotype and environment on wheat starch properties
rn. Mr. Michael Turner. Mr. Colin Bailey and Assoc. Prof. Balwant Singh. My thanks extend for help from my colleagues: Michael Nelson. Jingwen Cai andEFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of Docto Effects of genotype and environment on wheat starch properties ec High Schools, and Can Tho University and KULeuven.iii1 am grateful to my father Nghĩa Nhan and my mother Anil 1 ran who have raised and educated me. Thanks to my brother Tin Nhan and my sister ưyen Nhan for encouragement. Thanks to my son Vinh Tien Nhan who always brings me happiness and dynamic Effects of genotype and environment on wheat starch properties to motivate me to study.1 would like to thank very much my beloved wife Ngoc My Van l.e who always shares, understands, supports and encourages me toEffects of genotype and environment on wheat starch properties
study.All are very important for me to complete this thesis.Thank you all.Minh Tri NIIANABSTRACTVariability of starch between wheat varieties grown inEFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of Docto Effects of genotype and environment on wheat starch properties thesis is to increase the understanding of environmental factors that influence variability of starch structural characteristics, and in turn starch properties that affect wheat grain quality.Five commercial Australian wheat varieties grown in five different geographic regions of Australia in two y Effects of genotype and environment on wheat starch properties ears were used to isolate and examine starch properties. Starch structure at the nanometer scale was characterized by techniques of High Performance AEffects of genotype and environment on wheat starch properties
nion Exchange Chromatography (HPAEC) for amylopectin chain length distribution. X-ray diffraction (XRD) for intensity of crystallinity, and small -angEFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of Docto Effects of genotype and environment on wheat starch properties rch granule size distribution, starch-granule proteins, and amylose content. Starch functionality was studied by a series of test including swelling power, thermal properties by Differential Scanning Calorimetry (DSC), and pasting properties by Rapid Viscosityanalyser (RVA), gel syneresis, gel stren Effects of genotype and environment on wheat starch properties gth and in vin o enzymatic digestibility.This study showed that variety was the main influence on starch granule size distribution, starch characterisEffects of genotype and environment on wheat starch properties
tics (amylose content, starch-granule proteins and amylopectin chain length distribution, starch swelling power). Growth location influenced strongly EFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of Docto Effects of genotype and environment on wheat starch properties y, DSC thermal properties and gel hardness. Interactions between genotype (varieties) and environmental factors (year, location) contributed significantly to crystallinity, flour swelling power and pasting viscosity. Growing conditions (soil type, soil nutrients, temperature, rainfall and the number Effects of genotype and environment on wheat starch properties of clear days) had highly significant correlations with wheat grain yield, protein and starch content, relative crystallinity. Environmental temperatEffects of genotype and environment on wheat starch properties
ure parameters before flowering and during grain filling had relationships with DSC thermal temperaturesVof starch and hardness of starch gels. The teEFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of Docto Effects of genotype and environment on wheat starch properties illing had negative correlations with breakdown viscosity and gel hardness.With regards to in vitro enzymatic digestibility, variety controlled the initial digestibility of native starch granules, and the rapidly digestible starch (RDS) of cooked and cooled starch. Growth location affected strongly Effects of genotype and environment on wheat starch properties the more advanced digestibility of native starch, the slowly digestible starch (SDS) and resistant starch (RS) of cooked and cooled starch. The in vitEffects of genotype and environment on wheat starch properties
ro enzymatic digestibility of native starch had some significant correlations with growing conditions. The biphasic kinetic model described adequatelyEFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of Docto Effects of genotype and environment on wheat starch properties tion data of native starch. The in vitro enzymatic digestibility of native starch was related to amylose content, starch-granule proteins (SGP). lamellar crystallinity, granule size and thermal properties. The in Vitro enzymatic digestibility of cooked and cooled had correlations with amylose conten Effects of genotype and environment on wheat starch properties t, the very short and short chain lengths of amylopectin, starch swelling power (SSP). granule size and pasting viscosityStorage time was the main facEffects of genotype and environment on wheat starch properties
tor influencing retrogradation (syneresis and hardness) and in vitro enzymatic digestibility of stored starch gels. However, variety also contributed EFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of Docto Effects of genotype and environment on wheat starch properties Amylose content and size of starch granules played an important role in syneresis and in vitro enzymatic digestibility of stored starch gels. Short chains (DP 7 - 14) of amylopectin had a positive correlation on syneresis and in vitro enzymatic digestibility of stored starch gels after storage 8 to Effects of genotype and environment on wheat starch properties 17 days.In conclusion, this thesis showed that environment mainly influenced grain quality; both genotype and environment affected strongly starch grEffects of genotype and environment on wheat starch properties
anule characteristics; and all of genotype, environment and processing influenced significantly starch functional properties, including in vitro enzymEFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of DoctoEFFECTS OF GENOTYPE AND ENVIRONMENT ON WHEAT STARCH PROPERTIESMinh Tri NHANA thesis submitted in fulfilment of the requirement for the degree of DoctoGọi ngay
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