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Structural and mechanistic studies of post translationally modified peptieds and proteins

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Nội dung chi tiết: Structural and mechanistic studies of post translationally modified peptieds and proteins

Structural and mechanistic studies of post translationally modified peptieds and proteins

Structural and mechanistic studies of post-translationally modified peptides and proteinsA thesis submitted for the degree of Doctor of Philosophyby T

Structural and mechanistic studies of post translationally modified peptieds and proteins Thi Thanh Nha TranM. Sc. (Chemistry)from theDepartment of ChemistryTHE UNIVERSITY‘/ADELAIDE41883CONTENTSAcknowledgments...............................

....................................iStatement or Originality.........................................................iiAbstract...................... Structural and mechanistic studies of post translationally modified peptieds and proteins

..................................................iiiChapter 1: Mass spectrometry based proteomics: an overview........................11.1Introductio

Structural and mechanistic studies of post translationally modified peptieds and proteins

n to mass spectrometry' based proteomics .....................11.2Mass spectrometry of peptides and proteins ..............................21.3Elcctro

Structural and mechanistic studies of post-translationally modified peptides and proteinsA thesis submitted for the degree of Doctor of Philosophyby T

Structural and mechanistic studies of post translationally modified peptieds and proteins OF 2 mass spectrometer................................................81.6Peptide protein preparation for mass spectrometry'......................111.

6.1Protein separation................................................121.6.2Protein digestion ................................................131.6.3P Structural and mechanistic studies of post translationally modified peptieds and proteins

eptide clean up prior to MS .....................................141.7Peptide, protein sequencing by mass spectrometry'.......................141.8Pos

Structural and mechanistic studies of post translationally modified peptieds and proteins

itive ion mass spectrometry..........................................151.8.1Proton mobility and fragmentation pathways of low-energy collision-induced

Structural and mechanistic studies of post-translationally modified peptides and proteinsA thesis submitted for the degree of Doctor of Philosophyby T

Structural and mechanistic studies of post translationally modified peptieds and proteins Negative ion mass spectrometry..........................................191.9.1Amide backbone cleavages..........................................191.9

.2Side-chain induced backbone cleavages.............................211.9.3P’ (beta prime) fragmentation.....................................231.9.4Ch Structural and mechanistic studies of post translationally modified peptieds and proteins

aracteristic side-chain fragmentations..........................24110 Post-translational modification.......................................25Chapter

Structural and mechanistic studies of post translationally modified peptieds and proteins

2: Investigation of phosphorylated peptides by negative ion mass spectrometry ........................................................................

Structural and mechanistic studies of post-translationally modified peptides and proteinsA thesis submitted for the degree of Doctor of Philosophyby T

Structural and mechanistic studies of post translationally modified peptieds and proteins proteome

Structural and mechanistic studies of post-translationally modified peptides and proteinsA thesis submitted for the degree of Doctor of Philosophyby T

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