Study on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cells

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Nội dung chi tiết: Study on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cells

Study on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cells

DOCTORAL THESISStudy on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cellsThe United Graduate School of Veterinary Sci

Study on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cells ienceYamaguchi UniversityDAO VAN CUONG43160Tabic of contentsAbstractiiiGeneral introduction1Chapter 1: MARCKS is involved in incthvhncrcury-induccd de

crease in cellviability and nitric oxide production in F.A.hy926 cells6 71Abstract2Introduction83. 3. 5.Materials and methods 3.1.Cell viability assay Study on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cells

9 3.2.Cell cycle analysis by flow cytometry10 3.3.Wound healing assay11 3.4.Tube formation assay11 3.5.Measurement of NO productionII 3.6.Transfection

Study on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cells

of siRNA and plasmid DNA12 3.7.Western blotting12 3.8.Statistical analysis13 Results 4.1.Effect ofMel 1g on endothelial cell viability14 4.2.Effect o

DOCTORAL THESISStudy on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cellsThe United Graduate School of Veterinary Sci

Study on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cells by calpain during toxic effects of niethylniercury between SH-SY5Y and EA.hy926 cells1. Abstract312Introduction323Materials and methods3.1.Cell cultur

e343.2.Cell viability assay343.3.Measurement of intracellular Ca'mobilization353.4.Western blotting353.5.Knock-down of MARCKS expression..363.6.Statis Study on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cells

tical analysis364Results4.Ỉ. Suppression of MeHg-induced decrease in cell viability by calpam inhibitors374.2.Calcium mobilization and calpain activat

Study on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cells

ion induced by MeHg 374.3.Suppression of MeHg-induced decrease in MARCKS expression bycalpain inhibitors384.4.Effect of caipain inhibitors on MeHg-ind

DOCTORAL THESISStudy on effects and mechanisms of methylmercury toxicity on neuronal and endothelial cellsThe United Graduate School of Veterinary Sci