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Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.full

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Nội dung chi tiết: Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.full

Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.full

https://khothuvien.AEM Accepted Manuscript Posted Online 14 May 2021Appl Environ Microbiol doi:10.1128/AEM.00200-21Copyright 2021 American Society for

Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.fullr Microbiology. All Rights Reserved.1234567s91011121314151617181920212223Title: Amino acid analog induces stress response in marine SynecwcoccusAuthor

s: Dana E. Michels’. Brett Lomemck*'. Tsui-Fen Chou', Michael J. Sweredoskr. Alexis Pasulka1*■Biological Sciences Department. California Polytechnic S Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.full

tate University. San Luis Obispo. c A 93407. USA;Proteome Exploration Laboratory. Beckman Institute. Division of Biology and Biological Engineering. C

Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.full

alifornia Institute of Technology. Pasadena. CA 91125. USA’Corresponding author: apasulka'q calpolv.eduABSTRACTCharacterizing the cell-level metabolic

https://khothuvien.AEM Accepted Manuscript Posted Online 14 May 2021Appl Environ Microbiol doi:10.1128/AEM.00200-21Copyright 2021 American Society for

Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.fulline food web dynamics and biogeochemical cycling. The time-selective proteome-labeling approach, bioorthogonal noncanonical amino acid tagging (BONCAT

). has potential to provide insight into differential allocation of resources at the cellular level, especially when coupled with proteomics. However, Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.full

the application of this technique in marine phytoplankton remains limited. We demonstrate that the marine cyanobacteria Synedxxvcctii sp. and two gro

Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.full

ups of eukaryotic algae take up the modified amino acid L-homopropargylglycme (HPG). suggesting BONCAT can be used to detect translationally active ph

https://khothuvien.AEM Accepted Manuscript Posted Online 14 May 2021Appl Environ Microbiol doi:10.1128/AEM.00200-21Copyright 2021 American Society for

Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.fullechococcus ip. cells grown with HPG revealed a physiological shift m nitrogen25metabolism, general protein stress, and energy production, indicating a

potential limitation for26the use of BONCAT in understanding the cell-level response of Sy»echữcocciis ip. to27environmental change. Variability in H Applied and Environmental Microbiology-2021-Michels-AEM.00200-21.full

PG sensitivity between algal groups and the impact of

https://khothuvien.AEM Accepted Manuscript Posted Online 14 May 2021Appl Environ Microbiol doi:10.1128/AEM.00200-21Copyright 2021 American Society for

https://khothuvien.AEM Accepted Manuscript Posted Online 14 May 2021Appl Environ Microbiol doi:10.1128/AEM.00200-21Copyright 2021 American Society for

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