Preservation of Methanogenic Cultures to Enhance Anaerobic Digest
➤ Gửi thông báo lỗi ⚠️ Báo cáo tài liệu vi phạmNội dung chi tiết: Preservation of Methanogenic Cultures to Enhance Anaerobic Digest
Preservation of Methanogenic Cultures to Enhance Anaerobic Digest
Marquette Universitye-Publications@MarquetteDissertations (1934 -)Dissertations, Theses, and Professional ProjectsPreservation of Methanogenic Culture Preservation of Methanogenic Cultures to Enhance Anaerobic Digestes to Enhance AnaerobicDigestionUjwal H. BhattadMarquette UniversityFollow this and additional works at: https://epublications.marquette.edu/dissertations_mu dr Part of the Environmental Engineering CommonsRecommended CitationBhattad, Ujwal H., 'Preservation of Methanogenic Cultures to Enhance Anaer Preservation of Methanogenic Cultures to Enhance Anaerobic Digestobic Digestion' (2012).Dissertations (1934 ■). 209.https://epublications.marquette.edu/dissertations_mu/209PRESERVATION OF METHANOGENIC CULTURES TO ENPreservation of Methanogenic Cultures to Enhance Anaerobic Digest
HANCE ANAEROBIC DIGESTIONbyUjwal H. Bbattad, ME.A Dissertation submitted to the Faculty of the Graduate School. Marquette University.in Partial FulfilMarquette Universitye-Publications@MarquetteDissertations (1934 -)Dissertations, Theses, and Professional ProjectsPreservation of Methanogenic Culture Preservation of Methanogenic Cultures to Enhance Anaerobic DigestOBIC DIGESTIONUjwal H. Bhattad. ME.Marquette University. 2012rhe use of anaerobic biotechnology is increasing as a sustainable process to treat various organic wastes. Methanogens convert organic COD into Gill and play the key role to drive thermodynamically unfavorable biochemical fermentation reac Preservation of Methanogenic Cultures to Enhance Anaerobic Digesttions and keep the digestion process steady and efficient. Progressive understanding of anaerobic microbiology with digester functionality may help toPreservation of Methanogenic Cultures to Enhance Anaerobic Digest
develop efficient, customized methanogenic cultures to enhance anaerobic bioprocesses. Preservation of methanogenic cultures via drying would be a coMarquette Universitye-Publications@MarquetteDissertations (1934 -)Dissertations, Theses, and Professional ProjectsPreservation of Methanogenic Culture Preservation of Methanogenic Cultures to Enhance Anaerobic Digestty to O; toxicity and drying, and very limited work is reported on their preservation. The work described herein involves preservation and subsequent storage of various methanogenic cultures in oxic conditions as well as applications to improve performance of anaerobic digesters and standardize labo Preservation of Methanogenic Cultures to Enhance Anaerobic Digestratory testing.Five methanogenic cultures were customized under different growth conditions. The cultures were preserved using freeze- and heat-dryingPreservation of Methanogenic Cultures to Enhance Anaerobic Digest
, and subsequently stored for short and long periods in the presence of air. Their activity was then assayed by measuring specific methanogenic activiMarquette Universitye-Publications@MarquetteDissertations (1934 -)Dissertations, Theses, and Professional ProjectsPreservation of Methanogenic Culture Preservation of Methanogenic Cultures to Enhance Anaerobic Digestrary and qPCR techniques were used to identify and quantify methanogenic communities before and after drying. The usefulness of preserved cultures was examined to bioaugment transiently upset anaerobic digesters and as seed inocula tor a standard laboratory lest, the biochemical methane potential (B Preservation of Methanogenic Cultures to Enhance Anaerobic DigestMP) assay. The effect of bioaugmentation was correlated with methanogenic community structure using the DGGE molecular fingerprinting technique.All cuPreservation of Methanogenic Cultures to Enhance Anaerobic Digest
stomized methanogenic cultures were significantly active even after handling, drying and subsequent storage in the presence of air, suggesting methanoMarquette Universitye-Publications@MarquetteDissertations (1934 -)Dissertations, Theses, and Professional ProjectsPreservation of Methanogenic Culture Preservation of Methanogenic Cultures to Enhance Anaerobic Digest culture developed in the presence of limited Q? exhibited higher methanogenic activity than cultures developed in strict anaerobic conditions regardless of the drying method employed. Glucose as a protective agent resulted in higher methanogenic activity, more so in freeze drying than heat drying. Preservation of Methanogenic Cultures to Enhance Anaerobic DigestSome methanogenic community members were found to be more tolerant to drying stress than others. Dried methanogenic cultures were found to be viable oPreservation of Methanogenic Cultures to Enhance Anaerobic Digest
ptions to use as a bioaugment to improve treatment efficiency of anaerobic digesters after toxic upset and for the BMP assay.iACKNOWLEDGEMENTSUjwal H.Marquette Universitye-Publications@MarquetteDissertations (1934 -)Dissertations, Theses, and Professional ProjectsPreservation of Methanogenic Culture Preservation of Methanogenic Cultures to Enhance Anaerobic Digestl that without their contribution it would have been impossible to reach this level.Formost. I owe my deepest gratitude to my research advisor Dr. Danial Zitomer who made this dissertation possible and because of him. my graduate experience has been one that I will cherish forever. I am greatly than Preservation of Methanogenic Cultures to Enhance Anaerobic Digestkful to his excellent guidance, caring, patience, and providing me with an excellent atmosphere for doing research. He provided me a full-time researcPreservation of Methanogenic Cultures to Enhance Anaerobic Digest
h assistantship through out my study which helped me a lot to focus on my research and not to worry on my finance. His mentorship was a key in providiMarquette Universitye-Publications@MarquetteDissertations (1934 -)Dissertations, Theses, and Professional ProjectsPreservation of Methanogenic Culture Preservation of Methanogenic Cultures to Enhance Anaerobic Digest. I am confident that these professional qualities will help me in my future success.I am also grateful to my research committee members for theữ continuous support, encouragement and suggestions to improve the quality of my research. I am thankful to Dr. Jim Maki for helping me to develop my backgr Preservation of Methanogenic Cultures to Enhance Anaerobic Digestound in molecular biology and providing his lab facility to do some advance molecular work. It was a wonderful experience working under his guidance,Preservation of Methanogenic Cultures to Enhance Anaerobic Digest
also a great relief talking to him during many stressful situations in dissertation writing. It was also a great pleasure for having Dr. Michael SwitzMarquette Universitye-Publications@MarquetteDissertations (1934 -)Dissertations, Theses, and Professional ProjectsPreservation of Methanogenic Culture Preservation of Methanogenic Cultures to Enhance Anaerobic Digest delighted to thank Mike Dollhopf. (Lab Manager. Water Quality Center. Marquette University) for assisting me in lab experimental set-up. and keeping track of lab supplies which helped a lot in completing the research (asks on time without any interceptions. I found him a great person on professiona Preservation of Methanogenic Cultures to Enhance Anaerobic Digestl and personal level.I am very thankful to Keerthi Cherukuri. (Graduate student. Department of Biological Sciences. Marquette University) for teachingPreservation of Methanogenic Cultures to Enhance Anaerobic Digest
, and working collaboratively onMarquette Universitye-Publications@MarquetteDissertations (1934 -)Dissertations, Theses, and Professional ProjectsPreservation of Methanogenic CultureMarquette Universitye-Publications@MarquetteDissertations (1934 -)Dissertations, Theses, and Professional ProjectsPreservation of Methanogenic CultureGọi ngay
Chat zalo
Facebook