Ebook Practical biochemistry: Part 2
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Ebook Practical biochemistry: Part 2
Determination of Total Protein and Albumin15A. DETERMINATION OF SERUM TOTAL PROTEIN AND ALBUMINProteins are polymers of L-amino acids. There are numer Ebook Practical biochemistry: Part 2rous proteins in our body concerned with different functions. Here we shall discuss plasma/serum proteins. Plasma and serum are both fluid part of the blood. Plasma is the supernatant, obtained upon addition of an anticoagulant to the blood where as serum is the supernatant obtained when the plain b Ebook Practical biochemistry: Part 2lood Specimen is allowed to clot. Hence serum is devoid of fibrinogen where as plasma contains fibrinogen.Plasma/serum proteins comprise a complex mixEbook Practical biochemistry: Part 2
ture of different proteins. The important proteins present in the plasma/serum are:1Albumin2Globulin3Conjugated proteins such as lipoproteins4FibrinogDetermination of Total Protein and Albumin15A. DETERMINATION OF SERUM TOTAL PROTEIN AND ALBUMINProteins are polymers of L-amino acids. There are numer Ebook Practical biochemistry: Part 2 - albumin (bilirubin), transferrin (iron)3Hormone function • TSH4Coagulation - fibrinogen5Defense - antibodies6Nutritional - albumin7Catalytic - enzyme proteinsLiver is involved in the synthesis of albumin, fibrinogen, prothrombin , other clotting factors and other several proteins coming under alp Ebook Practical biochemistry: Part 2ha and beta globulins.Determination of total protein method used: Biuret method.Specimen: Serum or plasma.Principle: Proteins form purple coloured comEbook Practical biochemistry: Part 2
plex with cupric ions in alkaline solution.The biuret test is given by those substances containing two carbamyl groups (CONH) joined either directly oDetermination of Total Protein and Albumin15A. DETERMINATION OF SERUM TOTAL PROTEIN AND ALBUMINProteins are polymers of L-amino acids. There are numer Ebook Practical biochemistry: Part 2 should have a minimum of two peptide bonds to give copper coordination complex that impart violet color to test mỉxhưeReagents Required1 Sodium chloride 0.9% (Normal saline)2Biuret reagent3 Protein standard: 5 g%ProcedureSet tubes labeled as T, s and B For further steps see Fig. 15A-1.Determination Ebook Practical biochemistry: Part 2 of Total Protein and Albumin 15Add -100 pl test serum -2.5 ml scdium chloride (0.9 g%) -3 ml buret reagentAdd -100 pl standard -2.4 ml sodium chloridEbook Practical biochemistry: Part 2
e (0 9 9%) -3 ml biuret reagentBAdd-2.5 ml sodium chloride (0.9 g%)-3 ml biuret reagentMix solutions as shown and keep them for 10 minutes at 37'C or Determination of Total Protein and Albumin15A. DETERMINATION OF SERUM TOTAL PROTEIN AND ALBUMINProteins are polymers of L-amino acids. There are numer Ebook Practical biochemistry: Part 2spectrophotometer.Determination of Total Protein and Albumin15A. DETERMINATION OF SERUM TOTAL PROTEIN AND ALBUMINProteins are polymers of L-amino acids. There are numerGọi ngay
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