Amino acid analysis protocols

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Amino acid analysis protocols

Methods in Molecular BiologyVOLUME 159Amino AcidAnalysis ProtocolsEdited by Catherine CooperNicolle Packer Keith Williams1____________________________

Amino acid analysis protocols _________________________________________Amino Acid AnalysisAn OverviewMargaret I. Tyler1.Importance and utilityAmino acids are found either in the fr

ee state or as linear chains in peptides and proteins. There are 20 commonly occurring amino acids in proteins, which are shown in Table 1. Amino acid Amino acid analysis protocols

analysis has an important role in the study of the composition of proteins, foods, and feedstuffs. Free amino acids are also determined in biological

Amino acid analysis protocols

material, such as plasma and urine, and in fruit juice and wine. When it is performed on a pure protein, amino acid analysis is capable of identifyin

Methods in Molecular BiologyVOLUME 159Amino AcidAnalysis ProtocolsEdited by Catherine CooperNicolle Packer Keith Williams1____________________________

Amino acid analysis protocols determine the most suitable enzymatic or chemical digestion method for further study of the protein. It is also a useful method for quantitating the a

mount of protein in a sample (see Chapter 2 in this volume) and can give more accurate results than colorimetric methods.2.Historical ViewThe earliest Amino acid analysis protocols

experiments on the acid hydrolysis of proteins were performed by Braconnot in 1820. in which concentrated sulphuric acid was used to hydrolyze gelati

Amino acid analysis protocols

n, wool, and muscle fibers (4). Various reagents for performing protein hydrolysis were tried over the next 100 years, with 6 M HC1 becoming the most

Methods in Molecular BiologyVOLUME 159Amino AcidAnalysis ProtocolsEdited by Catherine CooperNicolle Packer Keith Williams1____________________________

Amino acid analysis protocols s on an ion-exchange resin and quantitation of them using ninhydrin.More recently, high-performance liquid chromatographs (HPLCs) have been configured

for amino acid analysis. Some methods use postcolumn deriv-From: Methods in Molecular Biology, vol. 159: Amino Acid Analysis ProtocolsEdited by: c. C Amino acid analysis protocols

ooper. N. Packer, and K. Williams © Humana Press Inc.. Totowa. NJ12TylerTable 1Common Amino AcidsNameSymbolEssential for humans (1)3 letter1 letterAci

Amino acid analysis protocols

dic amino acidsAspartic acidAspDNoGlutamic acidGluENoNeutral amino acidsAlanineAlaANoAsparagineAsnNNoCysteineCysCNoGlutamineGinQNoGlycineGlyGNoIsoleuc

Methods in Molecular BiologyVOLUME 159Amino AcidAnalysis ProtocolsEdited by Catherine CooperNicolle Packer Keith Williams1____________________________