New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells
➤ Gửi thông báo lỗi ⚠️ Báo cáo tài liệu vi phạmNội dung chi tiết: New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells
New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells
MONASH UniversityNew polymeric nanoparticles to interrupt the ROS- and RNS-derived misregulation of cellsVan Nam DAOBPharm. (Hons). MPharmSc.A thesis New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells submitted for the degree of Doctor of Philosophy atMonash University in 2021Faculty of Pharmacy and Pharmaceutical SciencesMONASH UniversityNew polymeric nanoparticles to interrupt the ROS- and RNS- derived misregulation of cellsVan Nam DAOBPharm. (Hons)r MPharmSc.Supervisors:Dr. John Quinn Dr. Mic New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells hael Whittaker A/ Prof. Erica SloanPanel members:Dr. Angus Johnston Dr. Betty Exintaris Dr. Daniel PooleA thesis submitted for the degree of Doctor ofNew polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells
Philosophy at Monash University in 2021Faculty of Pharmacy and Pharmaceutical SciencesCopyright notice©Van Nam DAO (2021).I certify that I have made MONASH UniversityNew polymeric nanoparticles to interrupt the ROS- and RNS-derived misregulation of cellsVan Nam DAOBPharm. (Hons). MPharmSc.A thesis New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells to my work without the owner's permission.AbstractReactive oxygen species (ROS) and reactive nitrogen species (RNS) have critical roles in various biological processes and signaling pathways. Moreover, perturbations in ROS and RNS levels often occur with changes in metabolism and health disorders. T New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells he application of scavengers, which are capable of reducing ROS and RNS levels, could potentially protect cells from being damaged by these reactive mNew polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells
olecules. Further, manipulating ROS and RNS levels can also interrupt downstream communication between impaired cells and other surrounding tissue, asMONASH UniversityNew polymeric nanoparticles to interrupt the ROS- and RNS-derived misregulation of cellsVan Nam DAOBPharm. (Hons). MPharmSc.A thesis New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells ulfide compounds that mimic cellular defense pathways and offer inherent ROS scavenging activities is another potential approach to alleviate ROS levels. Further, the recent development of nanomedicine has enabled researchers to address issues associated with small molecular drugs, such as nonspecif New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells ic binding, low tissue permeation and short retention time.In this thesis, polymer-based scavengers for ROS and RNS have been developed which i) exploNew polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells
it advanced polymer design, li) contain novel reactive moieties, lii) scavenge ROS and/or RNS intracellularly, and iv) confer downstream effects in thMONASH UniversityNew polymeric nanoparticles to interrupt the ROS- and RNS-derived misregulation of cellsVan Nam DAOBPharm. (Hons). MPharmSc.A thesis New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells cal systems Recent advances in development of hydrogen sulfide and persulfide donors, as well as macromolecular ROS/RNS scavengers, are also described. Subsequently, in a series of experimental chapters, data are presented about four main categories of materials that have been developed: linear and New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells brush polymers bearing trisulfide moieties for releasing hydrogen sulfide and persulfides (Chapter 2 and Chapter 3). polymer carriers for delivering WNew polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells
-acetyl L-cysteine intracellularly (Chapter 4). star polymers for conjugating a small molecule antioxidant (TEMPO) (Chapter 5) and nitric oxide scavenMONASH UniversityNew polymeric nanoparticles to interrupt the ROS- and RNS-derived misregulation of cellsVan Nam DAOBPharm. (Hons). MPharmSc.A thesis New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells rther, the impact of the synthesised materials on cell viability, and their biological performance, i.e. ROS -RNS scavenging ability, were evaluated on several models, including a naturally elevated ROS level model which can simulate TME communication (a co-culture of fibroblasts and breast cancer c New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells ells, Chapters 2. 4 and 5). an externally stimulated oxidative stress model (HEK293 ưeated with H?o?. Chapter 3). and a naturally elevated RNS model (New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells
activated primary and cell line macrophages.Chapter 6) Where appropriate, other intracellular behaviour of the synthesised compounds was evaluated, inMONASH UniversityNew polymeric nanoparticles to interrupt the ROS- and RNS-derived misregulation of cellsVan Nam DAOBPharm. (Hons). MPharmSc.A thesis New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells ymer treatment were also examined, including ROS-associated cellular changes (collagen-1 and F-actm expression. Chapter 2). NO-llnked phagocytosis activity (Chaptei 6), or mitochondrial functions (superoxide anion production and co-localisation. Chapter 5). The materials developed in the thesis repr New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells esent novel entities for manipulating cellular signaling pathways, and are potentially applicable to diverse fields including the pharmaceutical, polyNew polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells
mer and material sciences, as well as oncology and pharmacologyPublications during enrolment1. Urquhart MC. Dao NV Ercole F. Boyd BJ, Davis TP, WhittaMONASH UniversityNew polymeric nanoparticles to interrupt the ROS- and RNS-derived misregulation of cellsVan Nam DAOBPharm. (Hons). MPharmSc.A thesis New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells tt. 2020:9(4 ):553-7.2 Dao NV. Ercole F. Kaminskas LM, Davis TP. Sloan EK. Whittaker MR. et al Trisulfide-bearing PEG brush polymers donate hydrogen sulfide and ameliorate cellular oxidative stress. Biomacromolecules. 2020 21(12):5292-305.3. Dao NV. Ercole F. Urquhart MC. Kaminskas LM. Nowell CJ. Da New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells vis TP. et al. Trisulfide linked cholesteryl PEG conjugate attenuates intracellular ROS and collagen-1 production in a breast cancer co-culture model.New polymeric nanoparticles to interrupt the ros and rns derived misregulation of cells
Biomaterials Science. 2021. DOI 10 1039/D0BM01544JMONASH UniversityNew polymeric nanoparticles to interrupt the ROS- and RNS-derived misregulation of cellsVan Nam DAOBPharm. (Hons). MPharmSc.A thesis MONASH UniversityNew polymeric nanoparticles to interrupt the ROS- and RNS-derived misregulation of cellsVan Nam DAOBPharm. (Hons). MPharmSc.A thesisGọi ngay
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