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Nội dung chi tiết: Phẫu thuật điều trị động kinh thùy thái dương

Phẫu thuật điều trị động kinh thùy thái dương

Molecular Characterization of the Mechanisms of Adhesion of Helicobacter pylori', a Model of Environmental Regulation of Bacterial AttachmentbyMario H

Phẫu thuật điều trị động kinh thùy thái dương Huesca ContrerasA thesis submitted in conformity with the requirements for the degree of Doctor of Philosophy, Graduate Department of Molecular and Me

dical Genetics University of Toronto.Copyright by Mario Huesca Contreras 1999.1*1National Library of CanadaAcquisitions and Bibliographic Services395 Phẫu thuật điều trị động kinh thùy thái dương

Wellinglon StfOflt Ottawa ON K1A0N4CanadaBibliothèque nalionale du CanadaAcquisitions et services bibliographiques395. rue WelHnglonOttawa ON K1ACN4Ca

Phẫu thuật điều trị động kinh thùy thái dương

nadaYou'IWOur &te Mr» r»tẠ’0rc*The author has granted a nonexclusive licence allowing the National Library of Canada to reproduce, loan, distribute or

Molecular Characterization of the Mechanisms of Adhesion of Helicobacter pylori', a Model of Environmental Regulation of Bacterial AttachmentbyMario H

Phẫu thuật điều trị động kinh thùy thái dương nor substantial extracts from it may be printed or otherwise reproduced without the author’s permission.L’auteur a accordé une licence non exclusive

pennettant à la Bibliothèqưe nationale du Canada de reproduce, prêter, distribuer OU vendre des copies de cette these SOUS la forme de microfiche/film Phẫu thuật điều trị động kinh thùy thái dương

, de reproduction sur papier OU sur format électronique.L’auteur conserve la propriété du droit d’auteur qui protege cette these. Ni la thèse ni des e

Phẫu thuật điều trị động kinh thùy thái dương

xtraits substantiels de celle-ci ne doivent être imprimés OU autrement reproduits sans son autorisation.0-612-41025-0CanadaMolecular Characterization

Molecular Characterization of the Mechanisms of Adhesion of Helicobacter pylori', a Model of Environmental Regulation of Bacterial AttachmentbyMario H

Phẫu thuật điều trị động kinh thùy thái dương tor of Philosophy 1999Department of Molecular and Medical Genetics, University of Toronto.Incubation of acid-treated Helicobacter pylori with lipid re

ceptors in vitro, showed a change in the pattern of binding usually observed at neutral pH. The major lipid species recognized at pH 2.0 and pH 2.5, w Phẫu thuật điều trị động kinh thùy thái dương

hich is the pH of the stomach lumen, were sulfatides (sulfogalactosylceramide, SGC), one of the most abundant glycolipids of the human stomach mucus.T

Phẫu thuật điều trị động kinh thùy thái dương

he suspicion that the SGC binding of H. pylori was a stress induced response is supported by the finding of a similar change in binding specificity wh

Molecular Characterization of the Mechanisms of Adhesion of Helicobacter pylori', a Model of Environmental Regulation of Bacterial AttachmentbyMario H

Phẫu thuật điều trị động kinh thùy thái dương y the inclusion of protein synthesis inhibitors, or by incubation of acid-treated H. pylori with anti-Hsp antibodies. Expression of Hsps in surface ex

tracts of H. pylori, and bacterial surface reactivity with anti-Hsp antibodies, correlated with the change in glycolipid binding specificity.Previous Phẫu thuật điều trị động kinh thùy thái dương

experiments in our laboratory which showed binding of heat shock proteins members of the Hsp70 family to sulfatide, led the investigation of the role

Phẫu thuật điều trị động kinh thùy thái dương

of the H. pylori Hsp70 (DnaK) in the acid stress induction of SGC binding. The H. pylori DnaK gene was cloned and sequenced, and its level of transcri

Molecular Characterization of the Mechanisms of Adhesion of Helicobacter pylori', a Model of Environmental Regulation of Bacterial AttachmentbyMario H

Phẫu thuật điều trị động kinh thùy thái dương s, was induced in H. pylori, exposed to 42°c and pH 2.5. Analysis of metabolically labeled proteins, induced by incubation of H. pylori at acidic cond

itions (pH 2.5), showed a typical post-stress pattern of protein synthesis. Two major electrophoretic bands induced by these conditions corresponded t Phẫu thuật điều trị động kinh thùy thái dương

o the heat shock proteins Hsp60 (GroEL) and DnaK.Preliminary characterization of PE-binding adhesins which correspond to a second phase of H. pylori c

Phẫu thuật điều trị động kinh thùy thái dương

olonization, that is the attachment to the epithelial cells surface and the entry into intercellular spaces, identified several proteins including the

Molecular Characterization of the Mechanisms of Adhesion of Helicobacter pylori', a Model of Environmental Regulation of Bacterial AttachmentbyMario H

Phẫu thuật điều trị động kinh thùy thái dương (BSA) and TB66, a protein identified in Mycobacterium tuberculosis.iii

Molecular Characterization of the Mechanisms of Adhesion of Helicobacter pylori', a Model of Environmental Regulation of Bacterial AttachmentbyMario H

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