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Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

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Nội dung chi tiết: Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer43891NGUYEN THI NH1ENACKNOWLEDGMENTSThe

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer studies were carried out at the Laboratory of Animal Reproduction. Faculty of Bioscience and Bioindustry. Tokushima University. This thesis would not

have been possible without the inspiration and support of several great individuals who have contributed towards shaping this thesis. 1 want to thank Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

all those people who made this work possible.Foremost. 1 would like to express my special appreciation and thanks to the Japan International Cooperat

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

ion Agency. SATREPS project supported my scholarship.I also would like to express my sincere gratitude to my supervisor Prof. Dr. Takcshige Otoi. Assi

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer43891NGUYEN THI NH1ENACKNOWLEDGMENTSThe

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer e, motivation, and immense knowledge. Their guidance helped me in all the lime of research and writing of this thesis. I wish to express my gratitude

to the members of my committee. Prof, llidcaki Nagamunc and Prof. Yoshiro Uto for comments on this study.My sincere thanks also go to Mrs. Masahiro Ni Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

l. Dr. Zhao Namula, Dr. Takayuki Hirano. Dr. l.e Anh Quynh. Dr. Qingyi Lin and all present members in the Laboratory of Animal Reproduction. Faculty o

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

f Bioscience and Bioinduslry (Ishii campus. Tokushima University) for helping me to achieve this thesis. Without their precious support, it would not

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer43891NGUYEN THI NH1ENACKNOWLEDGMENTSThe

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer g me spiritually throughout writing this thesis and my life in general.CONTENTSCHAPTER 1. INTRODUCTION............................................11.1

.Introduction..................................................11.2.Objectives of the Thesis......................................2CHAPTER 2. LITERATU Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

RE REVIEW.......................................42.1. Cryobiology...................................................52.2Somatic cell nuclear transfer

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

(SC.'NT)........................102.3Interspecics SCNT (iSCNT).....................................11SUPPLEMENTED WITH CHLOROGENIC ACID...............

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer43891NGUYEN THI NH1ENACKNOWLEDGMENTSThe

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer .153.3Materials and methods.........................................173.3.1In vitro maturation.......................................183.3.2In vitro f

ertilization....................................193.3.3I VC and assessment of blastocyst quality.................193.3.4Experimental design........... Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

............................213.3.5Statistical analysis......................................223.4Results.............................................

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

..........233.4.1Experiment 1...............................................233.4.2Experiment 2...............................................243.4.3E

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer43891NGUYEN THI NH1ENACKNOWLEDGMENTSThe

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer VELOPMENT OF ZONA PELLUC IDA-FREE PORCINE ZYGOTES INDIVIDUALLY CULTURED AFTERVITRIFICATION......................................................314.1A

bstract.......................................................31 Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer

Studies on preservation of porcine zygotes for embryo production by interspecies somatic cell nuclear transfer43891NGUYEN THI NH1ENACKNOWLEDGMENTSThe

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