Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
➤ Gửi thông báo lỗi ⚠️ Báo cáo tài liệu vi phạmNội dung chi tiết: Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
Micropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts lm Leibniz Universităt Hannover zur Erlangung des GradesDoktor der Gartenbauwissenschaften(Dr. rer. hort.)genehmigte DissertationvonNgocTuan Pham, Master of Agriculture (Vietnam) geboren am 04.12.1977 in Dalat / Vietnam2017Referent: Prof. Dr. Traud Winkelmann Korrcfcrcnt: Prof. Dr. Thomas DcbcnerTag Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts der Promotion: 20. 07. 2017“Learning is not attained by chance, it must be sought for With ardor and attended to with diligence. ”Abigail AdamsAbstraMicropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
ctSpecies and hybrids belonging to the genus Jugtans. e. g.. J. regia and J. nigra X J. regia (- J. X intermedia), deliver highly valuable timber thatMicropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts or plantation forestry is of great interest. Because aulovcgclalivc propagation of Jugtans is difficult to be applied, in vitro culture techniques could enable the production of high-quality clones. However, the Jugians genus is largely recalcitrant in terms of micropropagation. The reasons for the Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts difficulties include (I) the mature stale of the oriels, (2) the general presence of endophytes, probably already in zygotic embryos (3) the slow growMicropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
th in vitro resulting in low propagation rates, and (4) difficulties in rooting of the microcuttings. Thus, the aim of this study was to develop a micMicropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts s for a multiclonal variety of .7. X intermedia. A second objective was the isolation and characterization of endophytic bacteria from in-vitro material which could help lo improve micropropagation techniques of Jugtans clones and the plant let quality.Interspecific hybrid walnut genotypes of the FI Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts offspring originating from open pollination of a single hybridogenic J. nigra tree in the nursery of Anton Scholl were identified using DNA markers (Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
simple sequence repeals - SSRs). In lotah 55 Jugtans hybrid genotypes, established in vitro from embryo axes, were propagated using an optimized tissuMicropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts oots was achieved with 4.4 uM BA and 0.2 jtM IBA in Rugini (Rugini 1984) and DKW (Driver and Kuniyuki 1984) media, resulting in clone-depending propagation rates from 2.5 to 5.4 per subculture. The optimal gelling agent for shoot cultures was Kobe agar. The highest rooting percentages (50 to 73%) we Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts re obtained with a two step rooting protocol (1. induction phase in DKW medium with 12 uM IB A within five days in darkness; 2. root expression underMicropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
light in a mixture of a gelified ^'Concentrated DKW medium and vermiculite). The survival rates after the acclimatization were 75 to 93%.To obtain infMicropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts tributing pollen and compared to reference data sets. The results of paternity analyses were discussed regarding potential mating preferences of this particular hybridogenic J. nigra tree.Bacterial endophytes in Jugtans in vitro shoot cultures were analyzed using two different methods: (I) With a cu Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts lture-dependent approach eight morphologically varying isolates were detected by I6S rRNA sequencing. The findings include Moraxelia sp.» Bre-vundimonMicropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
as sp., Acinelobacier sp., Pseudomonas sp. and Roseomonas sp. (2) With a culture-independent approach seventeen libraries were established from an ampMicropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts a was delected in all analyzed samples, some of which arc known as plant growth promoting bacteria, such as Pseudomonas sp., F.rwinia sp.. Burkholderia sp.. Petomonas sp.. and Sphingomonas sp. The genus Moraxetta sp. was identified in the genotypes with higher propagation rates. This finding is a fi Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts rst step towards future inoculation experiments to prove the proposed beneficial effects during micropropagation (if Jugtans genotypes.Key words: JuglMicropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
ans X intermedia, micropropagation, endophytesZusaninienfassung (Abstract in German language)Arten und Hybriden der Gaming Juglans, Z.B. J. regia und Micropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts ehrung qualitativ hochwertiger Bãume fur eine spãtere waldbauliche Oder plantagenartige Verwendung. Da eine autovegetative Vermehrung von Nussbaumen kaum moglich ist. konnten Zn-v/zro-Kulturtechniken die Produktion hochwertiger Klone ermoglichen. Die Gattung Jt!glans envies sich jedoch in Bezug auf Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts die Mikrovennehrung als schwierig. Die Griinde fur solche Schwierigkeiten liegen (1) in der Altersphase der Ausgangsbaume, (2) dem generellen AuftreteMicropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
n von Endophyten, vermutlich bereits in zygotischen Embryonen. (3) den niedrigen In-vitro-Vermehningsraten sowie (4) Schwierigkeiten in der BewurzelunMicropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts ums an J. X irtterwecfai-Genotypen, welches die anschlieBende Selektion hervorragender Genotypen fur eine Hybridnuss-Mehrklonsorte ermoglicht. Ein zweites Ziel war die Isolierung und Charakterisierung endophytischer Bakterien aus /»-vửrớ-Material, welche zur Verbesserung der Mikrovermehrung von Jugi Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts ans eingesetzt werden kõnnten.Fur die Selektion und Mikrovermehrung neuer Nusshybriden (J. X intermedia) konnten Fl-Nachkommen aus freier Abbliite einMicropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
es hybridisierenden Schwarznussbaumes (J. nigra) in der Baumschule Anton Schott genutzt und uber DNA-Marker ( Simple-Sequence-Repeats SSRs) identifizMicropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts gs-und Bewurzehingsraten sowie einer einfachen Akklimatisierung mikrovermehrt. Die beste Sprossstreckung und Bildung neuer Achselknospen wurde mit 4.4 U.M BA und 0.2 pM IBA in Rugini- (Rugini 1984) und DKW- (Driver and Kuniyuki 1984) Medien erreicht. wobei klonabhiingige Vermelirungsaten von 2,5 bis Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts 5,4 erzielt wurden. Das oprimale Geliennittel fur die Sprosskulturen war Kobe-Agar. Die hochsten Bewurzelungsraten (50 bis 73 %) wurden mit einem ZweMicropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
i-Schritt-Bewurzelungsprotokoll erzielt (1. funf Tage Induktionsphase in DKW-Medium mil 12 pM IBA im Dunkeln, 2. Expressionsphase im Licht in einem GeMicropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts r die genetische Vielfalt der Nusshybriden ZU erhalten. wurden populationsgenetische Daten fiir die gesamte Nachkommenschaft als auch den Pollenbei-trag erhoben und mil Referenzdatensatzen verglichen. Die Ergebnisse von Vaterschafts-analysen wurden in Bezug auf die potentielle Bestaubungspraferenz d Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts ieser besonderen hybridisierenden Schwarznuss diskutiert. Bakterielle Endophyten in Jugians-Sprosskulturen wurden Ober zwei verschiedene Methoden unteMicropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
rsucht: (1) Mit einem kulmrabhangigen Ansatz wurden acht morphologisch unterscheidbare Isolate gewonnen und Ober 16S rRNA-Sequenzierung identifiziert.Micropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts wurden aus der amplifizierten 16S rRNA von 51 Pflanzen-DNA-Proben siebzehn Sequenzbibliotheken erstellt. Mil Hilfe der Illumina MiSeq-Sequenzienmg konnten mehr als 85 % der gesamten Bakterienpopulation erfasst werden. von denen einige als pilanzenwachstunisiordernd bekannt sind. wie Pseudomonas sp., Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts Envinia sp.. Burkhoideria sp.. Pelomonas sp. und Sphingomonas sp. In Genotypen mil hoher /rt-vữrớ-Vennehrungsrate wurde Moraxeìỉa sp. identifiziert.Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts
Dieses Ergebnis ist ein erster Schritt in Richtung kiinftiger Inokulationsversuche. uni mogliche positive Effekte auf die Mikrovermehrung von Jwg/aMs-Micropropagation and Identification ofBacterial Endophytes in Interspecific Hybrid WalnutsVon der Naturwissenschaftlichen Fakultat derGottfried Wilhel Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts .................................................IZusammenfassung (Abstract in German language)............................... IIIndex of contents...........................................................IllAbbreviations.................................................................V1General intr Micropropagation and identification of bacterial endophytes in interspecific hybrid walnuts oductionGọi ngay
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