Synthetic Feedback Loop for Increasing Microbial Biofuel Producti
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Synthetic Feedback Loop for Increasing Microbial Biofuel Producti
University of VermontScholarWorks (a) UVMGraduate College Dissertations and ThesesDissertations and Theses2013Synthetic Feedback Loop for Increasing M Synthetic Feedback Loop for Increasing Microbial Biofuel ProductiMicrobial Biofuel Production Using a BiosensorMary HarrisonL'ninirsify of VermontFollow this and additional works at: https://scholarworks.uvm.edu/graddisRecommended CitationHarrison, Mary, 'Synthetic Iwdback Loop for Increasing Microbial Biofuel Production Using a Biosensor* (20B). Graduate College Synthetic Feedback Loop for Increasing Microbial Biofuel Producti Diííertationí and Thestí. 104.https://scholarworkcuvmedu /graddis/104This thesis K brought to you for free and open access by the Dissertations and ISynthetic Feedback Loop for Increasing Microbial Biofuel Producti
hese. at SchoiarWodj. sió ƯVM. It has been accepted for UICỈUS1OC1 ,n Graduate College Dissertations and theses by an authorized administrator ot SchoUniversity of VermontScholarWorks (a) UVMGraduate College Dissertations and ThesesDissertations and Theses2013Synthetic Feedback Loop for Increasing M Synthetic Feedback Loop for Increasing Microbial Biofuel ProductiNG A BIOSENSORA Thesis PresentedbyMary HarrisontoThe Faculty of the Graduate CollegeofThe University of VermontIll Partial Fulfillment of the Requirements for the Degree of Master of Science Specializing in Biomedical EngineeringOctober. 2012Accepted by the Faculty of the Graduate College, The Unive Synthetic Feedback Loop for Increasing Microbial Biofuel Productirsity of Vermont, in partial fulfillment of the requirements for the degree of Master of Science, specializing in Biomedical Engineering.Thesis ExaminSynthetic Feedback Loop for Increasing Microbial Biofuel Producti
ation Committee:____________________________________Adv i sorMary Dunlop, Ph.D.Jason Bates. Ph.D.____________________________________ChairpersonMattheUniversity of VermontScholarWorks (a) UVMGraduate College Dissertations and ThesesDissertations and Theses2013Synthetic Feedback Loop for Increasing M Synthetic Feedback Loop for Increasing Microbial Biofuel Productin cellulose and convert It to biofuel. However, tins production is limited by the toxicity of the biofuel to the organism that IS producing It. Therefore, to increase yields, microbial biofuel tolerance must be increased. Tolerant strains of bacteria use a wide range of mechanisms to counteract the Synthetic Feedback Loop for Increasing Microbial Biofuel Productidetrimental effects of toxic solvents. Previous research demonstrates that efflux pumps are effective at increasing tolerance to various biofuels. HowSynthetic Feedback Loop for Increasing Microbial Biofuel Producti
ever, when overexpressed, efflux pumps burden cells, which hinders growth and slows biofuel production. Therefore, the toxicity of the biofuel must beUniversity of VermontScholarWorks (a) UVMGraduate College Dissertations and ThesesDissertations and Theses2013Synthetic Feedback Loop for Increasing M Synthetic Feedback Loop for Increasing Microbial Biofuel Productiack loop to control efllux pump expression so that it is proportional to the concentration of biofuel present. In this way. the biofuel production rate will be maximal when the concentration of biofuel is low because the cell does not expend energy expressing efflux pumps when they are not needed. A Synthetic Feedback Loop for Increasing Microbial Biofuel Productidditionally, the microbe IS able to adapt to toxic conditions by triggering the expression of efflux pumps, which allows it to continue biofuel producSynthetic Feedback Loop for Increasing Microbial Biofuel Producti
tion. The mathematical model shows that this feedback loop increases biofuel production relative to a model that expresses efflux pumps at a constant University of VermontScholarWorks (a) UVMGraduate College Dissertations and ThesesDissertations and Theses2013Synthetic Feedback Loop for Increasing M Synthetic Feedback Loop for Increasing Microbial Biofuel Producti system can use feedback to adjust to the actual production rate. To complement the mathematical model, we also constructed a whole cell biosensor that responds to biofuel by expressing a fluorescent reporter protein from a promoter under the control of the sensor. Synthetic Feedback Loop for Increasing Microbial Biofuel ProductiUniversity of VermontScholarWorks (a) UVMGraduate College Dissertations and ThesesDissertations and Theses2013Synthetic Feedback Loop for Increasing MGọi ngay
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