Ebook Textbook of biochemistry (7th edition): Part 2
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Ebook Textbook of biochemistry (7th edition): Part 2
CHAPTER 28Plasma ProteinsChapter at a GlanceThe reader will be able to answer questions on the following topics:Plasma proteinsElectrophoresisAlbumin, Ebook Textbook of biochemistry (7th edition): Part 2, functions, clinical significanceHypoalbuminemiaGlobulins, alpha, beta, gamma>Transport proteins in bloodr Acute phase proteins in bloodr Ceruloplasmin>Alpha-1 antitrypsinA Clotting factorsTotal blood volume is about 4.5 to 5 liters in adult human being. Tf blood is mixed with an anticoagulant and Ebook Textbook of biochemistry (7th edition): Part 2centrifuged, the cell components (RBC and WBC) are precipitated. The supernatant IS called plasma. About 55-60% of blood is made up of plasma.I.If bloEbook Textbook of biochemistry (7th edition): Part 2
od is withdrawn without anticoagulant and allowed to clot, allcr alxml 2 hours liquid jxnlion is separated from the clot. This dcfibrinalcd plasma is CHAPTER 28Plasma ProteinsChapter at a GlanceThe reader will be able to answer questions on the following topics:Plasma proteinsElectrophoresisAlbumin, Ebook Textbook of biochemistry (7th edition): Part 2The plasma proteins consist of albumin (3.5 to 5 g/dL), globulins (2.5 - 3.5 g/dL) and fibrinogen (200 400 mg’dl.). The albumin : globulin ratio is usually between 1.2:1 to 1.5:1.iv.Almost all plasma proteins, except immunoglobulins are synthesized in liver. Plasma proteins are generally synthesized Ebook Textbook of biochemistry (7th edition): Part 2 on membrane-bound polyribosomes. Most plasma proteins are glycoproteins.V. In laboratory, separation can be done by salts. Thus, fibrinogen IS precipEbook Textbook of biochemistry (7th edition): Part 2
itated by 10° o and globulins by 22% concentration of sodium sulfate, jlmmonium sulfate will precipitate globulins at half saturation and albumin at fCHAPTER 28Plasma ProteinsChapter at a GlanceThe reader will be able to answer questions on the following topics:Plasma proteinsElectrophoresisAlbumin, Ebook Textbook of biochemistry (7th edition): Part 2ntitated by Bromo cresol green (BCG) method, m winch the dye is preferentially bound with albumin, and the color is estimated colonmetrically.ELECTROPHORESISTil clinical laboratory, electrophoresis is employed regularly for sejiaration of scrum proteins. The tenu electrophoresis refers to the moveme Ebook Textbook of biochemistry (7th edition): Part 2nt of charged particles through an electrolyte when subjected to an electric field The details are given in Chapter 35. Normal and abnormal electrophoEbook Textbook of biochemistry (7th edition): Part 2
retic patterns are shown in Figures 28.1 and 28.2.Chapter 28: Plasma Proteins 379Normal Patterns and Interpretationsi.In agar gel electrophoresis, norCHAPTER 28Plasma ProteinsChapter at a GlanceThe reader will be able to answer questions on the following topics:Plasma proteinsElectrophoresisAlbumin, Ebook Textbook of biochemistry (7th edition): Part 2obulin : 8-12%Gamma globulin : 12 22%ii.Albumin has the maximum and gamma globulin has the minimum mobility in the electrical field.lii. Gamma globulins contain the antibodies (immunoglobulins). Most of the alpha-1 fraction is made up of alpha-1 antitrypsin. Alpha-2 band is mainly made lip by alpha- Ebook Textbook of biochemistry (7th edition): Part 22 macroglobulin. Beta fraction contains low density lipoproteins.Fig. 28.1 A: Serum electrophoretic patterns. 1 Normal pattern; 2 Multiple myeloma (MEbook Textbook of biochemistry (7th edition): Part 2
hand} between |i and y region; 3 =Chronic infection, broad based Increase in y region; general increase in al and a2 bands; 4 = Nephrotic syndrome; hyCHAPTER 28Plasma ProteinsChapter at a GlanceThe reader will be able to answer questions on the following topics:Plasma proteinsElectrophoresisAlbumin, Ebook Textbook of biochemistry (7th edition): Part 2 paraproteinsAbnormal Patterns in Clinical DiseasesVarious abnormalities can be identified in the electrophoretic pattern (Figs 28.1A and B).CHAPTER 28Plasma ProteinsChapter at a GlanceThe reader will be able to answer questions on the following topics:Plasma proteinsElectrophoresisAlbumin,Gọi ngay
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