PROBLEM SOLVING TESTS IN MOLECULAR CELL

➤ Gửi thông báo lỗi ⚠️ Báo cáo tài liệu vi phạm

Loại tài liệu: PDF

Số trang: 66 Trang

Tài liệu: ✅ ĐÃ ĐƯỢC PHÊ DUYỆT

TẢI VỀ

Nội dung chi tiết: PROBLEM SOLVING TESTS IN MOLECULAR CELL

PROBLEM SOLVING TESTS IN MOLECULAR CELL

PROBLEM-SOLVING TESTS IN MOLECULAR CELL BIOLOGYJozsef SzeberenyiUniversity of Pécs Medical School 2015.SZECHENYITERVTechnical assistanceZita ArvaiMóni

PROBLEM SOLVING TESTS IN MOLECULAR CELL ika VecsernyésWith the permission of the International Union of Biochemistry and Molecular Biology.Supported by a grant from the European Union (TÁMOP

-4.1.1.C-13/l/KONV-2014-0001).^SZECHENYITERVA FM* M u— ll —CONTENTSElectrophoretic behavior of hemoglobin variants1Real-time polymerase chain reaction PROBLEM SOLVING TESTS IN MOLECULAR CELL

5Targeted gene disruption10The effect of bisulfite treatment on genomic DNA15Analysis of the cell cycle by flow cytometry18a 1-Antitrypsin deficiency:

PROBLEM SOLVING TESTS IN MOLECULAR CELL

An example for a protein folding disease22Vectorial transport in intestinal epithelial cells32Expression cloning of the erythropoietin receptor36The

PROBLEM-SOLVING TESTS IN MOLECULAR CELL BIOLOGYJozsef SzeberenyiUniversity of Pécs Medical School 2015.SZECHENYITERVTechnical assistanceZita ArvaiMóni

PROBLEM SOLVING TESTS IN MOLECULAR CELL oprotein51Signal transduction in Philadelphia chromosome positive leukemia cells56SZECHENYITERV——SZECHENYITERV1ELECTROPHORETIC BEHAVIOR OF HEMOGLOBIN

VARIANTSTerms to be familiar with before you start to solve the testHemoglobins * erythrocytes * reticulocytes ammo acids * a and p globin chains * el PROBLEM SOLVING TESTS IN MOLECULAR CELL

ectrophoresis * isoelectric point * sickle cell anemiaThe experimentHemoglobin A (HbA), the predominant adult hemoglobin in our red blood cells is a v

PROBLEM SOLVING TESTS IN MOLECULAR CELL

ital protein responsible for the transport of oxygen. It is synthesized in immature reticulocytes and carries out its function in mature erythrocytes.

PROBLEM-SOLVING TESTS IN MOLECULAR CELL BIOLOGYJozsef SzeberenyiUniversity of Pécs Medical School 2015.SZECHENYITERVTechnical assistanceZita ArvaiMóni

PROBLEM SOLVING TESTS IN MOLECULAR CELL ited diseases caused by mutations in a and p globin genes. Two of the most common hemoglobin variants are HbS (expressed in red blood cells of patient

s that suffer from sickle cell anemia) and HbC (causing a milder condition). In both disorders, the mutation affects codon 6 in p globin mRNA: the glu PROBLEM SOLVING TESTS IN MOLECULAR CELL

tamic acid (Glu) at position 6 in the p globin chain is replaced by valine (Vai) or lysine (Lys) in HbS and HbC, respectively (the formulae of these a

PROBLEM SOLVING TESTS IN MOLECULAR CELL

mino acids are shown in Figure 1.Glu

PROBLEM-SOLVING TESTS IN MOLECULAR CELL BIOLOGYJozsef SzeberenyiUniversity of Pécs Medical School 2015.SZECHENYITERVTechnical assistanceZita ArvaiMóni