Effects of mannan oligosasaccharide on immune function and disease resistance in pigs
➤ Gửi thông báo lỗi ⚠️ Báo cáo tài liệu vi phạmNội dung chi tiết: Effects of mannan oligosasaccharide on immune function and disease resistance in pigs
Effects of mannan oligosasaccharide on immune function and disease resistance in pigs
EFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of the Effects of mannan oligosasaccharide on immune function and disease resistance in pigs e requirements for the degree of Doctor of Philosophy in Animal Sciences in the Graduate College of theUniversity of Illinois at Urbana-Champaign. 2010Urbana. IllinoisDoctoral Committee:Professor James E. Pettigrew. Chair and Director of ResearchProfessor Keith w. KelleyProfessor Rodney w. JohnsonPr Effects of mannan oligosasaccharide on immune function and disease resistance in pigs ofessor William G. Van Alstine. Purdue UniversityAssociate Professor Hans H. SteinABSTRACTThe 3 studies described below demonstrate the effects of matEffects of mannan oligosasaccharide on immune function and disease resistance in pigs
man oligosaccharide (MOS) on immune function and disease resistance in pigs. Study 1 evaluated whether MOS in both in vivo and in vitro systems regulaEFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of the Effects of mannan oligosasaccharide on immune function and disease resistance in pigs from MOS-fed pigs produced less tumor necrosis factor'd (TNF-a) (P < 0.01) and more IL-10 (P 0.051) than AM<|> from the control-fed pigs. Dietary MOS did not affect AMộ-produced cytokines induced by polyinosinic:polycytidylic acid (Poly I:C). When applied in vitro, MOS suppressed LPS-induced TNF-a Effects of mannan oligosasaccharide on immune function and disease resistance in pigs (P < 0.001), but enhanced LPS-induced IL-10 (P < 0.05). Further. TNF-a production by AMỘ stimulated with LPS (P<0.05) or Poly I:C (P< 0.001) was supprEffects of mannan oligosasaccharide on immune function and disease resistance in pigs
essed by a mannan-rich fraction (MRF). In order to leant if MOS interacts with LPS receptors. AMỘ were cultured with Polymyxin B. an inhibitor of LPS-EFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of the Effects of mannan oligosasaccharide on immune function and disease resistance in pigs S to regulate cytokine production in the absence of LPS. When added in vitro, both MOS and MRF were also able to regulate constitutive production of TNF-d in the absence of LPS. Study 2 determined if various levels of dietary MOS affect growth and serum cytokine levels in nursery pigs. No effect of Effects of mannan oligosasaccharide on immune function and disease resistance in pigs MOS on growth was found. There were no differences in serum levels of TNF'd and IL-10. although these levels changed over time. Study 3 showed that MOEffects of mannan oligosasaccharide on immune function and disease resistance in pigs
S altered nursery pigs’ immune response to a porcine reproductive and respiratory syndrome virus (PRRSV). Infection of PRRSV reduced pig performance aEFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of the Effects of mannan oligosasaccharide on immune function and disease resistance in pigs (Pl) and tended to improve feed efficiency. Tn infected pigs. MOS reduced fever at d 7 PI (P < 0.01) and serum TNT-a at d 14 PI (P -0.06). The gene expression profile in peripheral blood mononuclear cells and bronchoalveolar lavage fluid cells at d 7 PT was characterized by using microarray and real Effects of mannan oligosasaccharide on immune function and disease resistance in pigs lime Rl-PCR. rhe MOS X PRRSV interaction altered the gene expression in the above leukocytes (P < 0.05). In peripheral blood mononuclear cells. MOS iEffects of mannan oligosasaccharide on immune function and disease resistance in pigs
ncreased the gene expression of pattern recognition receptors, cytokines, and intracellular signaling molecules in uninfected pigs, but reduced the geEFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of the Effects of mannan oligosasaccharide on immune function and disease resistance in pigs omote a cytotoxic T cell immune response by enhancing M11CI mRNA expression, but reduce the expression of complement system-associated molecules and 2',5'-oligoadenylate synthetase-1. The downregulation of inflammatory' responses regulated by MOS at d 7 PI was associated with several important canon Effects of mannan oligosasaccharide on immune function and disease resistance in pigs ical pathways such as triggering receptor expressed on myeloid cells-1 signaling, hypoxia signaling. IL-4 signaling, macropinocytosis signaling, and pEffects of mannan oligosasaccharide on immune function and disease resistance in pigs
erhaps the alternative activation of macrophages, in summary, MOS is a potent immunomodulator in both vitro and in vivo systems. Dietary inclusion of EFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of the Effects of mannan oligosasaccharide on immune function and disease resistance in pigs tem.Key words; Alveolar macrophages and cytokine secretion; disease resistance; immunomodulalion; mannan oligosaccharide; pigs; PRRSViiiDEDICATEDTOMY MOTHER. WIFE. AND SON FOR ALL THEIR SPIRITUAL SUPPORTSMost of us. swimming against the tides of trouble the w orld knows nothing about, need only a bi Effects of mannan oligosasaccharide on immune function and disease resistance in pigs t of praise or encouragement - and we w ill make the goal.Jerome p. FleishmanivACKNOWLEDGEMENTSI owe my deepest gratitude to my Ph.D. supervisor. ProfEffects of mannan oligosasaccharide on immune function and disease resistance in pigs
essor James E. Pettigrew, whose constant encouragement and support throughout my study enabled me to learn and understand an interesting area of reseaEFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of the Effects of mannan oligosasaccharide on immune function and disease resistance in pigs s in my research. He continually provided me with wise advice, skillful mentoring, and great kindness.I am indebted to all advisory committee members for their whole-hearted instruction, persistent assistance, and extraordinary devotion. I am especially grateful to Professor Keith w. Kelley and Prof Effects of mannan oligosasaccharide on immune function and disease resistance in pigs essor Rodney w. Johnson, whose experience and broad insights enriched my research and knowledge in the field of nutritional immunology. Their sound inEffects of mannan oligosasaccharide on immune function and disease resistance in pigs
terpretation and productive comments on the study results are invaluable. It is also an honor for me to work with Professor William G. Van Alstine. whEFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of the Effects of mannan oligosasaccharide on immune function and disease resistance in pigs rateful to Assoc. Professor Hans H. Stein for his willingness to join my supervisory committee as well as for his valuable comments on the format of this dissertation.I would like to thank the laboratory technicians for supporting me in many different faculties. Particularly, the assistance of .ToEl Effects of mannan oligosasaccharide on immune function and disease resistance in pigs la Barnes from laboratory analyses to experimental work in the field has been highly appreciated. A special thanks is also given to Jing Chen for herEffects of mannan oligosasaccharide on immune function and disease resistance in pigs
willingness to help familiarize me with cell cultures at the initial stage of my research.Special thanks are offered to Alltech and its staff for finaEFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of the Effects of mannan oligosasaccharide on immune function and disease resistance in pigs o Dr. Kail Dawson. Dr. Cohn Moran, and Alltech's vice president. Mr. Aidan Connolly for their kind help, incessant encouragement, and valuable discussions.It is a pleasure to thank all my colleagues, friends, administrative staff, and farm crew who made this research possible. A sincere gratitude is Effects of mannan oligosasaccharide on immune function and disease resistance in pigs offered to my labmates for not only their multiple contributions to the completion of this dissertation but also their sharing weal and woe throughouEffects of mannan oligosasaccharide on immune function and disease resistance in pigs
t my study.EFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of theEFFECTS OF MANNAN OLIGOSACCHARIDE ON IMMUNE FUNCTION AND DISEASE RESISTANCE IN PIGSBYTUNG MINH CHEDISSERTATIONSubmitted ill partial fulfillment of theGọi ngay
Chat zalo
Facebook