THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETII
➤ Gửi thông báo lỗi ⚠️ Báo cáo tài liệu vi phạmNội dung chi tiết: THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETII
THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETII
THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR)PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIA Senior Honors ThesisbyMARY JEANELL WILSONSubmitted to t THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIthe office of Honors Programs& Academic Scholarships Texas A&M UniversityIn partial fulfillment of the requirements of (heUNIVERSITY UNDERGRADUATE RESEARCH FELLOWS38808Major: Biochemistry<- THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) ONIRON REGULATION IN COX1ELLA BURNETiiA Senior Honors Thesis by THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIMARY JEANELL WILSONSubmitted to the office of Honors Programs & Academic Scholarships Texas A&M UniversityIn partial fulfillment for the designation oTHE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETII
fUNIVERSITY UNDERGRADUATERESEARCH FELLOWSApproved as to style and content by:James E. Samuel (Fellows Advisor)Edward A. 1'unkhouser (Executive DirectoTHE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR)PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIA Senior Honors ThesisbyMARY JEANELL WILSONSubmitted to t THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIanell Wilson Department of Biochemistry Texas A&M UniversityFellows Advisor: James E. SamuelDepartment of Microbial and Molecular PathogenesisCoxiella burnetii is a gram negative, obligate intracellular bacterium. It is the etiologic agent of Q fever in a variety of species including livestock, huma THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIns and arthropods. The bacterium infects the monocytes of its host and is encapsulated in the phagolysosome, an acidic vacuole meant to kill the bacteTHE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETII
rium, where it survives and replicates, c. burnetii must be able to acquire all the nutrients necessary for survival within this acidic environment, iTHE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR)PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIA Senior Honors ThesisbyMARY JEANELL WILSONSubmitted to t THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIron concentration must be maintained as a delicate balance.Too little iron and replication is impeded, while too much iron initiates theivproduction of oxygen radicals which are fatal to the cell. Ferric iron uptake Regulator (Fur) is responsible for the regulation of iron acquisition genes in many THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIgram negative bacteria. Fur acts as a transcriptional repressor of iron regulated genes. These genes have a sequence within their promoter region callTHE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETII
ed the “Fur box” that binds to the Fur protein when the protein is also bound to its co-repressor, Fe2*. In E. coll. Fur has been found responsible foTHE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR)PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIA Senior Honors ThesisbyMARY JEANELL WILSONSubmitted to t THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETII promoters with a highly conserved consensus sequence are part of a Fur regulon. Our goal is to characterize this regulon. Nineteen putative Fur boxes were identified in c. burnetii. Fifteen of these were cloned into the pBlue plasmid expressing beta-galactosidase. These plasmids were then co-transf THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIormed with a plasmid expressing Fur into an E.coii fur deletion strain. The p-galactosidase assay was then used to test promoter activity. Eleven of tTHE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR) PROTEIN ON IRON REGULATION IN COXIELLA BURNETII
hese promoters were evaluated. Three promoters, for open reading frames CBU0970, CBƯ0769 and feoB were found to be repressed in the presence of iron. THE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR)PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIA Senior Honors ThesisbyMARY JEANELL WILSONSubmitted to tTHE ROLE OF FERRIC IRON UPTAKE REGULATOR (FUR)PROTEIN ON IRON REGULATION IN COXIELLA BURNETIIA Senior Honors ThesisbyMARY JEANELL WILSONSubmitted to tGọi ngay
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