Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial mats
➤ Gửi thông báo lỗi ⚠️ Báo cáo tài liệu vi phạmNội dung chi tiết: Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial mats
Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial mats
1 Phosphorus deprivation responses and phosphonate utilization in a thermophilic2Synechococcus sp. from microbial mats34Melissa M. Adams8 b *, Maria R Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial matsR. Gomez-Garcia8, Arthur R. Grossman8, Devaki Bhaya856s Carnegie Institution for Science,7Department of Plant Biology,8Stanford, CA 94305910fc Department of Biology,llStanford University12Stanford, CA 943051314Running title: Pho regulon of a thermophilic Synechococcus sp.15Keywords: phosphate starva Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial matstion, Pho regulon, phn genes, methylphosphonate1617*Corresponding authorCarnegie Institution for Science,18260 Panama Street19Stanford, CA 9430520TelePhosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial mats
phone: (650) 325-152121Fax: (650) 325-685722E-mail: mmadamsKiPstanford.edu1123ABSTRACT24 The genomes of two closely related thermophilic cyanobacteria1 Phosphorus deprivation responses and phosphonate utilization in a thermophilic2Synechococcus sp. from microbial mats34Melissa M. Adams8 b *, Maria R Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial mats sequenced. An extensive suite of 27genes that are controlled by phosphate levels constitute the putative Pho regulon in these 28cyanobacteria. We examined physiological responses of an axenic Synechococcus OS-B', 29as well as transcript abundances of Pho regulon genes as the cells acclimated to 30p Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial matshosphorus-limiting conditions. Upon imposition of phosphorus deprivation, 3lSynechococcus OS-B' stopped dividing after 3-4 doublings and absorbance spPhosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial mats
ectra 32measurements indicated that the cells had lost most of their phycobiliproteins and 33chlorophyll a. Alkaline phosphatase activity peaked and r1 Phosphorus deprivation responses and phosphonate utilization in a thermophilic2Synechococcus sp. from microbial mats34Melissa M. Adams8 b *, Maria R Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial matsgenome of Synechococcus OS-B’ harbors a cluster of phn 36genes that are not present in Synechococcus OS-A.. The proteins encoded by the phn genes 37function in the transport and metabolism of phosphonates, which could serve as an 38alternative phosphorus source when exogenous phosphate is low. The p Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial matshn genes were up-39regulated within a day of eliminating the source of phosphate from the medium. However, 40the ability of Synechococcus OS-B' to utiPhosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial mats
lize methylphosphonate as a sole phosphorus 41source occurred only after an extensive period of exposure to the substrate. Once 42acclimated, the cell1 Phosphorus deprivation responses and phosphonate utilization in a thermophilic2Synechococcus sp. from microbial mats34Melissa M. Adams8 b *, Maria R Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial matsh respect 44to the ecophysiology of the microbial mats.22^INTRODUCTION46 Significant advances have been made in characterizing the diversity and 47ecophysiology ol microorganisms in the alkaline hot spring microbial mats OÍ Yellowstone 48National Park (4. 53). However, relatively little is known abo Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial matsut specific physiological 49adaplations and the acclimation responses of microbes in the mats with respect to nutrient 501imitation (1. 40, 42, 43). APhosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial mats
unicellular cyanobacterium Synechococcus sp. is the Sldominant, primary produc er in these hot spring microbial mats al temperatures above 50 c. 52an1 Phosphorus deprivation responses and phosphonate utilization in a thermophilic2Synechococcus sp. from microbial mats34Melissa M. Adams8 b *, Maria R Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial matsI'S sequence .^determination, it appears that Synechococcus ecotypes are delineated along environmental 55gradients, including temperature and light (9, 10). For instance, the Synechococcus OS-A 56(hereafter Syn OS-A) isolate is abundant in the high temperature region of the mat (58-5765°C) while th Phosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial matse Synechococcus OS-B’ (hereafter Syn OS-B’) isolate is abundant in the 581ower temperature regions (50-60°C). OS indicates that the organisms were isoPhosphorus deprivation responses and phosphonate utilization in a thermophilic Synechococcus sp. from microbial mats
lated from 59Qctopus Spring (9).1 Phosphorus deprivation responses and phosphonate utilization in a thermophilic2Synechococcus sp. from microbial mats34Melissa M. Adams8 b *, Maria R1 Phosphorus deprivation responses and phosphonate utilization in a thermophilic2Synechococcus sp. from microbial mats34Melissa M. Adams8 b *, Maria RGọi ngay
Chat zalo
Facebook